ZHANG Shi-jun,LIU Nian,SHENG Ai-wu,et al.Establishment of Calli-mediated Regeneration System in Curcuma longa[J].Northern Horticulture,2012,36(06):96-98.
姜黄愈伤组织再生体系的建立
- Title:
- Establishment of Calli-mediated Regeneration System in Curcuma longa
- 文章编号:
- 1001-0009（2012）06-0096-03
- Keywords:
- Curcuma longa; tissue culture; bud base; calli; regeneration
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以姜黄无菌芽的芽基为外植体，进行愈伤组织的诱导和无菌苗再生的研究。结果表明：在MS培养基中添加2，4-D或2，4-D与6-BA的激素组合能够诱导外植体的切口部位产生愈伤组织，但愈伤生长缓慢；在2，4-D和6-BA的激素组合中再加入TDZ，愈伤组织能够不断增殖生长：在MS+0.5 mg/L TDZ+0.5 mg/L BA+0.3 mg/L 2,4-D培养基中，愈伤的诱导率和生长量最高，诱导率达到80%，每个月愈伤生长量为148.6 mg；愈伤组织再分化出芽的适宜培养基为MS+0.5 mg/L TDZ+2.0 mg/L BA+0.2 mg/L NAA，再分化率为90%，外植体的平均出芽数为5.1。当试管苗长至5 cm时出瓶移栽，成活率可达90%以上。
- Abstract:
- A protocol was investigated for callus induction using bud base from tissue-cultured Curcuma longa explants.The results showed that MS media supplemented with 2,4-D or with 2,4-D+6-BA could successfully induce calli formation at cut positions of explants.However，the calli grew slowly.The optimal calli induction medium was MS+0.5 mg/L TDZ+0.5 mg/L BA+0.3 mg/L 2,4-D，which showed the highest induction rate and calli growing mass，with an inducing rate of 80% and growing mass of 148.6 mg per month.The optimal plantlet regeneration medium was MS+0.5 mg/L TDZ+2.0 mg/L BA+0.2 mg/L NAA，with a regeneration rate of 90% and the average shoot number of 5.1 per explant.Regenerated plantlets (5 cm at length) were transferred to soil in greenhouse.More than 90% of plantlets could survive.
参考文献/References:
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备注/Memo
第一作者简介：张施君（1974-），女，博士，副教授，现从事植物分子育种研究及教学等工作。E-mail：guanyehuazu@163.com。