[1]李晓娇,刘超,何春燕,等.粗梗稠李中花色苷的提取及抗氧化和抑菌活性研究[J].北方园艺,2021,(23):89-97.[doi:10.11937/bfyy.20211709]
　LI Xiaojiao,LIU Chao,HE Chunyan,et al.Study on Extraction and Antioxidative and Bacteriostatic Activities of Anthocyanins From Padus napaulensis[J].Northern Horticulture,2021,(23):89-97.[doi:10.11937/bfyy.20211709]

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粗梗稠李中花色苷的提取及抗氧化和抑菌活性研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年23 页码: 89-97 栏目: 出版日期: 2021-12-15

Title:: Study on Extraction and Antioxidative and Bacteriostatic Activities of Anthocyanins From Padus napaulensis

作者:: 李晓娇1; 刘超2; 何春燕1; 桂奇峰1; 侯洪波1; （1.保山学院资源环境学院，云南保山 678000；2.保山市质量技术监督综合检测中心，云南保山 678000）

Author(s):: LI Xiaojiao1; LIU Chao2; HE Chunyan1; GUI Qifeng1; HOU Hongbo1; 1.School of Resources and Environment,Baoshan University,Baoshan,Yunnan 678000;2.Baoshan Quality and Technical Supervision Comprehensive Testing Center,Baoshan,Yunnan 678000)

关键词:: 粗梗稠李; 花色苷; 抗氧化活性; 抑菌活性

Keywords:: Padus napaulensis; anthocyanin; antioxidant activities; anti-microbial activities

DOI:: 10.11937/bfyy.20211709

文献标志码:: A

摘要:: 以粗梗稠李成熟果实为试材，采用超声辅助法提取花色苷，通过单因素正交实验优化花色苷的提取工艺，并测定其抗氧化和抑菌活性，以期为粗梗稠李功能农产品的开发提供参考依据。结果表明：花色苷的最佳提取条件为乙醇体积分数50%、料液比为1∶60 g·mL-1、pH 10、超声时间50 min、提取温度40 ℃，该条件下，花色苷的平均提取量为0632 mg·g-1；粗梗稠李花色苷对·OH、DPPH·、ABTS+·、O·〖TX--*9〗2均有较好的清除能力，高浓度时对Fe2+有较好的螯合能力；对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌有较强的抑菌效果，其最低抑菌浓度（MIC）分别为1249、1249 mg·L-1和625 mg·L-1。

Abstract:: The anthocyanins in Padus napaulensis were extracted by ultrasonic assisted extraction,the extraction process was optimized by one-variate orthogonal experiments,and its antioxidant and bacteriostatic activities were determined,in order provide a reference for subsequent development of functional agricultural products using Padus napaulensis.The results showed that the optimum extraction conditions of the anthocyanin was as follows the ethanol volume fraction was 50%,the ratio of material to solution was 1∶60 g·mL-1,pH was 10,the ultrasound time was 50 minutes,and the extraction temperature was 40 ℃.Under this condition,the average amounts of anthocyanins extracted were 0632 mg·g-1.The anthocyanins had good scavenging ability on ·OH,DPPH·,ABTS+· and O·〖TX--*9〗2,and it had good chelating ability to Fe2+ at high concentration.It had strong antibacterial effect on E.coli,Staphylococcus aureus and Bacillus subtilis,and its minimum inhibitory concentration (MIC) was 1249 mg·L-1,1249 mg·L-1 and 625 mg·L-1,respectively.

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备注/Memo

第一作者简介：李晓娇（1985-），女，硕士，副教授，现主要从事天然产物研究与开发等研究工作。E-mail:lixiaojiao0622@163.com.责任作者：侯洪波（1979-），男，硕士，副教授，现主要从事天然产物研究与开发等研究工作。E-mail: hhb826@163.com.基金项目：云南省教育厅科学研究基金资助项目（2016ZZX221）；保山市中青年学术和技术带头人资助项目（bszqnxshjsdtr2016(03)）；保山市第八批创新团队资助项目（201911）；云南省2018年高校本科教育教学改革研究资助项目（JG2018225）。收稿日期：2021-04-20

更新日期/Last Update: 2022-03-16