MA Yangli,ZHANG Yuxiao,GUO Yanyin,et al.Effects of O2 Content on the Chlorophyll Degradation in Broccoli Heads Under Controlled Atmospheres[J].Northern Horticulture,2021,(23):98-105.[doi:10.11937/bfyy.20212248]
气调过程中O2含量对西兰花叶绿素降解的影响
- Title:
- Effects of O2 Content on the Chlorophyll Degradation in Broccoli Heads Under Controlled Atmospheres
- Keywords:
- broccoli; controlled atmospheres; yellowing; high O2; chlorophyll degradation
- 文献标志码:
- A
- 摘要:
- 以西兰花为试材，采用气调处理的方法，以自然空气为对照（CK），设置20%O2+20%CO2、50%O2+20%CO2、80%O2+20%CO2的气体比例，研究了20 ℃贮藏条件下O2对西兰花的贮藏品质和叶绿素降解的影响，阐明O2促进西兰花黄化的作用机制，以期为研究高O2条件下叶绿素的降解调控提供参考依据。结果表明：高O2比例降低了西兰花体内叶绿素、维生素C以及可溶性蛋白质含量；气调处理中O2比例提高能够促进叶绿素b还原酶编码基因（BoNYC1）、滞绿蛋白编码基因（BoSGR）、脱镁叶绿素酶编码基因（BoPPH）、脱镁叶绿酸a氧化酶编码基因（BoPaO）、红色叶绿素降解产物还原酶编码基因（BoRCCR)表达水平，尤其是BoSGR、BoPPH和BoRCCR相对表达量高峰提前出现，促进了叶绿素分解代谢，加速了西兰花黄化进程。其中，O2促进叶绿素降解过程中BoPPH基因表达可能是促进黄化的主要原因，SGR则通过互作影响BoPPH表达。
- Abstract:
- Taking broccoli as test materials,the air conditions of 20%O2+20%CO2,50%O2+20%CO2 and 80%O2+20%CO2 were employed as treatments,natural air was set as control (CK).During storage at 20 ℃,the storage quality of broccoli florets and the relative expression of genes related to chlorophyll degradation were determined.To explore the effects of O2 on the degradation of broccoli chlorophyll in controlled atmosphere.The results of this experiment could provide a theoretical basis for the regulation of chlorophyll degradation under high O2 conditions.The results showed that with the increase of O2 ratio,the chlorophyll b reductase encoding gene (BoNYC〖STBX〗1〖STBZ〗),the chlorophyll encoding gene (BoSGR),the pheophytinase encoding gene (BoPPH),the pheophorbide a oxidase encoding gene (BoPaO),red chlorophyll degradation product reductase encoding gene (BoRCCR) expression levels and their relative expression peaks appeared earlier,which promoted chlorophyll catabolism and accelerated the yellowing process of broccoli.Among them,O2 promoted the gene expression of BoPPH in the process of chlorophyll degradation,which may be the main reason for promoting yellowing,SGR interacted the expression of BoPPH.
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备注/Memo
第一作者简介：马阳历（1996-），男，硕士研究生，研究方向为农产品贮藏与加工。E-mail:942020639@qq.com.责任作者：郭衍银（1976-），男，博士，教授，现主要从事农产品贮藏与加工等研究工作。E-mail：guoyy@sdut.edu.cn.基金项目：国家自然科学基金面上资助项目（31671900）。收稿日期：2021-05-26