HUA Mingyan,SONG Lanfang,CUI Shaojie,et al.Effect of Potassium Fertilizer Treatment on Sugar Accumulation and Related Enzyme Activities in Strawberry Fruits[J].Northern Horticulture,2019,43(16):38-43.[doi:10.11937/bfyy.20190194]
钾肥处理对草莓果实糖积累及相关酶活性的影响
- Title:
- Effect of Potassium Fertilizer Treatment on Sugar Accumulation and Related Enzyme Activities in Strawberry Fruits
- Keywords:
- strawberry; potassium fertilizer; quality; enzymes activity
- 文献标志码:
- A
- 摘要:
- 以以色列品种“S3”草莓为试材，研究了不同钾肥处理对草莓蔗糖、果糖、葡萄糖含量及相关代谢酶的影响。结果表明：钾肥的施用可以促进草莓果实蔗糖、果糖、葡萄糖含量的增加，蔗糖是构成草莓可溶性糖的主要成分，果糖和葡萄糖含量基本相当,随着钾肥施用量的增大，施钾量为360 kg?hm-2时，可溶性糖含量最高；在花后42 d蔗糖合成酶（SS）活性最高，适量钾肥显著提高了草莓发育后期蔗糖合成酶的活性，其活性在草莓果实发育中后期与蔗糖、葡萄糖积累呈显著正相关，在果实发育前期与果糖积累呈负相关；蔗糖磷酸合成酶(SPS)在蔗糖合成积累中起着重要的作用,草莓成熟过程中，SPS活性总体呈上升趋势，与蔗糖含量呈极显著正相关；在草莓果实成熟过程中酸性转化酶（AI）、中性转化酶（NI）活性变化较大，总体在果实成熟过程中呈先下降后上升的趋势，且与果实中蔗糖含量呈负相关。钾离子对蔗糖代谢的相关酶具有重要的调节作用，适量的钾肥处理提高了蔗糖合成酶(SS)、蔗糖磷酸合成酶（SPS）、酸性转化酶（AI）、中性转化酶（NI）活性，从而提高生长期果实中蔗糖、果糖和葡萄糖积累量。
- Abstract:
- Israeli variety ‘S3’ strawberry was taken as the test material,the effects of different potassium fertilizer treatments on sucrose，fructose，glucose content and related metabolic enzymes in strawberry were studied.The results showed that the application of potassium fertilizer could promote increase of sucrose，fructose and glucose content in strawberry fruits.Sucrose was the main component of soluble sugar in strawberry fruits，and the content of fructose and glucose was basically the same.With the increase of potassium fertilizer，the sugar content increased and reached the maximum when the potassium was 360 kg?hm-2.The activity of sucrose synthase was the highest at 42 days after flowering，and the activity of sucrose synthase was significantly increased by potassium fertilizer at the later stage of strawberry development.Sucrose synthase activity was positively correlated with sucrose and glucose accumulation in the middle and late stage of strawberry fruit development，and negatively correlated with fructose accumulation in the early stage of fruit development.Sucrose phosphate synthase played an important role in the synthesis and accumulation of sucrose，which activity positively correlated with the sucrose content，the overall trend was upward.In the ripening of strawberry，the activity of acid invertase enzymes and neutral invertase enzymes changed greatly，which activity decreased first and then increased，and negatively correlated with the sucrose content in the fruits.Potassium ions played a key role in the regulation of sucrose metabolism-related enzymes.Proper potassium fertilizer could improve the activities of sucrose synthase，sucrose phosphate synthase and sucrose neutral invertase，thus increasing the accumulation of sucrose，fructose and glucose of fruit during growth period.
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备注/Memo
第一作者简介：华明艳（1983-），女，硕士，助理研究员，现主要从事草莓栽培及种苗繁育等研究工作。E-mail:huamingy1@163.com.责任作者：杨小玲（1968-），女，博士，研究员，现主要从事设施园艺作物栽培等研究工作。E-mail:yxlcacn@sina.com.基金项目：天津市蔬菜产业体系创新团队专项资助项目（ITTVRS2017019）；天津市种业科技重大专项资助项目（18ZXZYNC00130）；天津市农业科技成果转化与推广资助项目（201801020）；天津市林果产业体系创新团队专项资助项目（ITTFPRS2018005）。收稿日期：2019-03-07