HE Jiali,ZHOU Jiangtao,LYU Deguo.Effects of Cadmium on Growth，Cd Accumulation and Active Oxygen Metabolism of Malus baccata Borkh.Seedlings[J].Northern Horticulture,2019,43(16):53-61.[doi:10.11937/bfyy.20184470]
镉胁迫对山定子幼苗生长、镉积累及活性氧代谢的影响
- Title:
- Effects of Cadmium on Growth，Cd Accumulation and Active Oxygen Metabolism of Malus baccata Borkh.Seedlings
- Keywords:
- Malus baccata Borkh.; cadmium; accumulation; antioxidants
- 文献标志码:
- A
- 摘要:
- 以当年生山定子（Malus baccata Borkh.）幼苗为试材，采用水培法，研究了50 μmol?L-1的Cd胁迫处理10 d和20 d对山定子幼苗生长、Cd积累及活性氧代谢的影响，以期为山定子耐Cd生理机制的研究提供参考。结果表明：Cd胁迫显著抑制了山定子幼苗的生长发育，且随胁迫时间的延长抑制作用加剧。山定子根系Cd含量、富集总量以及生物富集系数（BCF）均大于茎和叶片，表明Cd主要在根中积累。Cd胁迫明显提高了各组织超氧阴离子（O?[TX-*4]2）、过氧化氢（H2O2）、丙二醛（MDA）、游离脯氨酸、可溶性酚和抗坏血酸含量。
- Abstract:
- Taking annual(Malus baccata Borkh.) seedling as test material，hydroponic method was adopted to study the effects of 50 μmol?L-1 Cd stress for 10 days and 20 days on the growth，Cd accumulation and active oxygen metabolism of seedling.The knowledge gained from this study provided a scientific basis for the study of physiological mechanism of Cd tolerance in M. baccata Borkh..The results showed that Cd stress significantly inhibited the growth and development of M. baccata Borkh.seedling，and the inhibitory effect was intensified with the prolongation of stress time.Cd concentration，total Cd and bio-concentration factor (BCF) in roots were all higher than those in stems and leaves，indicating that Cd was mainly accumulated in roots.Cd stress remarkably increased the contents of O?[TX-*4]2，H2O2，MDA，free proline，soluble phenolics and ascorbic acid.
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备注/Memo
第一作者简介：何佳丽（1987-），女，博士，讲师，硕士生导师，现主要从事苹果逆境生理学等研究工作。E-mail：hejiali1017@163.com.责任作者：吕德国（1967-），男，博士，教授，博士生导师，现主要从事果树栽培与生理生态等研究工作。E-mail：gszp2017@163.com.基金项目：国家自然科学基金资助项目（31501712）；辽宁省高等学校优秀人才支持计划资助项目（LJQ2015098）；十二五科技计划资助项目（2014BAD16B02-1）；沈阳农业大学引进人才科研启动资助项目（20153007）；国家现代农业产业技术体系建设专项资助项目（CARS-27）。收稿日期：2019-03-05