ZHENG Kai,DING Jiuling,CAI Hongyu.Tissue Culture and Rapid Micropropagation of Tillandsia stricta[J].Northern Horticulture,2016,40(20):106-109.[doi:10.11937/bfyy.201620027]
空气凤梨组培快繁技术
- Title:
- Tissue Culture and Rapid Micropropagation of Tillandsia stricta
- 文献标志码:
- A
- 摘要:
- 以斯垂科特空气凤梨（Tillandsia stricta）的种子为外植体，采用组织培养法，研究了不同基础培养基、植物生长调节剂种类及浓度对其生长的影响，以期筛选出适宜斯垂科特空气凤梨种子组培快繁的培养基配方。结果表明：以1/2MS为基础培养基，添加0.5 mg?L-1IAA，空气凤梨的发芽率和幼苗成活率高，幼苗生长健壮，色泽浓绿，真叶数多，比常规种子繁殖速度快2倍左右，幼苗成活率高1.45倍。
- Abstract:
- The seed of Tillandsia stricta was used as explant，effects of different basal medium，plant growth regulators types and concentration on growth were researched by using tissue culture method to select the optimal medium formula.The results showed that the basal medium was 1/2 MS supplemented IAA 0.5 mg?L-1，the germination rate and survival rate were high，seedling plants were stronger and dark green in leaf color and more leaf number.In this way，the propagation rate was 2 times and survival rate was 1.45 times more than conventional seed propagation.
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备注/Memo
第一作者简介：郑凯(1980-)，男，陕西西安人，博士研究生，副研究员，现主要从事植物资源收集及抗性生理等研究工作。E-mail：78056829@qq.com.责任作者：丁久玲(1978-)，女，河南许昌人，硕士，副研究员，现主要从事园林植物应用等研究工作。E-mail：317569479@qq.com.