LIANG Xiaomin,LUO Ganfeng.Key Technology for Tissue Culture of Bozhou White Peony Root Seedlings[J].Northern Horticulture,2016,40(20):102-105.[doi:10.11937/bfyy.201620026]
亳州芍药组织培养的关键技术
- Title:
- Key Technology for Tissue Culture of Bozhou White Peony Root Seedlings
- Keywords:
- Paeonia lactiflora Pall.; bulbils; explants collection time; surface sterilization method; plant tissue culture
- 文献标志码:
- A
- 摘要:
- 以亳州芍药鳞芽外植体为试材，对3个时间段（2月下旬至3月上旬、9月下旬至10月上旬、12月下旬至翌年1月上旬）的鳞芽进行了优化，并确定了亳州芍药组织培养的最佳取样时间；以2月下旬至3月上旬（第1阶段）鳞芽为试材，研究了5种鳞芽表面灭菌方法和IBA、NAA与BA组合的4因素2水平以及BA与KT组合的2因素2水平培养基组分筛选正交实验，以建立一套高效成熟的亳州芍药组织培养的快速繁殖技术体系。结果表明：2月下旬至3月上旬取样的亳州芍药鳞芽诱导成活率最高，采用0.13% HgCl2浸泡10 min对亳州芍药萌发芽进行2次灭菌效果最好；亳州芍药鳞芽最佳的增殖出芽分化培养基为1/2MS+1.0 mg?L-1 BA+0.5 mg?L-1 KT+3.0%蔗糖+0.7%琼脂。
- Abstract:
- The bulbils of Bozhou white peony root seedlings were used as explants for the trial of Bozhou peony tissue culture.Based on 3 stages of sampling，the best time for explants collection was optimized.Based on the bulbils collected in the last tenday of February and the first tenday of March，5 surface sterilizing conditions and 3 plant hormones IBA，NAA and BA at different levels of combinations with the experimental design of 4 factors and 2 levels orthogonal test were investigated.The BA and KT combinations with the experimental design of 2 factors and 2 levels orthogonal test were also optimized to establish the fast propagation technology for Bozhou white peony root seedlings.The results showed that the explants collected in the last tenday of February and the first tenday of March had the highest rate of induction.The best surface sterilizing condition was submersing the bulbils for 0.13% HgCl2 for 10 minutes and then repeated again.The optimized initiation culture medium for bulbils was 1/2 MS+1.0 mg?L-1 BA+0.5 mg?L-1 KT+3.0% sucrose+0.7% agar.
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备注/Memo
第一作者简介：梁小敏(1973-)，女，江西高安人，硕士，教授，研究方向为植物组织培养在种苗生产中的应用。E-mail:liangxm@sina.com.基金项目：江西省教育厅青年科学基金资助项目(GJJ09633)。