GAN Liping,RUAN Shenqing,ZENG Xiaolin.Tissue Culture System Screening of Actinidia chinensis cv.Hongyang ‘Sanxia Horg’[J].Northern Horticulture,2016,40(09):114-117.[doi:10.11937/bfyy.201609030]
“三峡虹”红阳猕猴桃组织培养体系的筛选
- Title:
- Tissue Culture System Screening of Actinidia chinensis cv.Hongyang ‘Sanxia Horg’
- Keywords:
- Actinidia chinensis cv.Hongyang; tissue culture; callus
- 文献标志码:
- A
- 摘要:
- 以红阳猕猴桃叶片、嫩茎、花蕾为外植体，MS为基本培养基，设置不同外植体消毒方法、不同的激素配方进行组织培养，比较了不同消毒剂对外植体的消毒效果、愈伤组织、不定芽的诱导情况，以及形成愈伤组织能力较强的外植体种类。结果表明：用75%酒精处理外植体30 s，再以1% NaClO消毒10 min，消毒效果达75.0%；诱导愈伤组织最适培养基为MS+1.0 mg/L ZT+0.1 mg/L NAA；诱导不定芽最适培养基为MS+1.0 mg/L 6-BA+0.1 mg/L NAA；用嫩茎作为外植体，诱导愈伤组织效果最好。
- Abstract:
- Taking the leaf,stems,buds of Hongyang kiwi fruit as explants，MS as basic medium，the effect of different disinfection methods，callus and bud induction ability of hormone formulations was studied.The results showed that the disinfectant method of 75% alcohol treatment explants 30 s，then 1% NaClO disinfection 10 min was the best with sterilizing effect reached 75.0%；callus optimum medium was MS+1.0 mg/L ZT+0.1 mg/L NAA while optimum medium for inducing adventitious buds was MS+1.0 mg/L 6-BA+0.1 mg/L NAA.The stems was the best explants for callus formation.
参考文献/References:
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备注/Memo
第一作者简介：甘丽萍（1979-），女，博士，副教授,现主要从事植物生理及生物技术等研究工作。E-mail：ganmei790717@163.com.