TAN Wu-ping,LIANG Yi,ZHANG Hong-wei,et al.Study on Embryo Regeneration System of Onion[J].Northern Horticulture,2014,38(16):93-95,96.
洋葱胚芽再生体系的研究
- Title:
- Study on Embryo Regeneration System of Onion
- 文章编号:
- 1001-0009(2014)16-0093-04
- Keywords:
- onion; callus; tissue culture
- 分类号:
- S663.2
- 文献标志码:
- A
- 摘要:
- 以洋葱胚芽为外植体材料，MS为基本培养基，研究了不同洋葱品种及不同激素组合对再生过程中洋葱愈伤组织诱导率的影响，建立洋葱胚芽再生体系。结果表明：“凤凰金球”洋葱品种愈伤诱导率最高；在4 mg/L2，4-D+1 mg/L 6BA的MS培养基上愈伤诱导率最高，为72.1%；在含1.0 mg/L TDZ+0.5 mg/L NAA的MS分化培养基上，愈伤分化率最高，为78.3%；在1/2MS+0.01 mg/L NAA培养基上可诱导生根，生根率100%；再生苗驯化移栽成活率达到 95%。
- Abstract:
- Taking the onion embryo as explants,the tilleredonion regeneration system was established with MS as the basic culture induction,the effect factors,such as different onion varieties and different combination of hormone treatment on the regeneration process was analiped.The results showed that ‘Fenhuang Jingiu’ onion varieties was suitable for culture induction with the highest callus induction rate;MS+4 mg/L 2-4，D+1 mg/L 6-BA was the most suitable for callus induction,the rate of callus induction reached 72.1%.Different ?varieties had the highest rate of callus with 72.1%;addition of 1.0 mg/L TDZ and 0.5 mg/L NAA to the MS medium promoted a the highest frequency of regeneration (78.3%);the medium which contained 1/2MS+0.01 mg/L NAA induced white strong roots,rooting rate reached 100%;the regenerated plantlets were domesticated and transplanted in greenhouse;the survival rate was 95%.
参考文献/References:
［1］Eady C C,Butler R C,Suo Y.Somatic embryogenesis and plant regenerationfrom immature embryo cultures of onion (Allium cepa L.)［J］.Plant Cell Reports,1998,18:111-116.
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备注/Memo
第一作者简介：谭武平（1989-），男，硕士研究生，研究方向为洋葱分子育种技术。E-mail：tanwuping1989@126.com.