YANG Wen-hong,HUANG Mei-juan,HUANG Hai-quan.Effects of NAA on Growth of ?Armillaria mellea of Gastrodia elata Blume in Zhaotong[J].Northern Horticulture,2013,37(09):169-171.
萘乙酸对昭通乌天麻野生蜜环菌生长的影响
- Title:
- Effects of NAA on Growth of ?Armillaria mellea of Gastrodia elata Blume in Zhaotong
- 文章编号:
- 1001-0009(2013)09-0169-03
- Keywords:
- Armillaria mellea; mycelium; rhizomorph; NAA; growth status
- 分类号:
- S 646.1+1
- 文献标志码:
- A
- 摘要:
- 以分离筛选后的昭通乌天麻野生蜜环菌为试材，以PDA为基本培养基，研究了不同浓度NAA对昭通乌天麻野生蜜环菌菌丝出现时间、菌索出现时间、菌索长度、生物量以及生长状况的影响。结果表明：处理4（5.0 μg/mL）的菌丝、菌索出现最快；处理1~5(0.5、1.0、2.5、5.0、10.0 μg/mL)菌索长度明显大于对照，处理6~9(25、50、100、250 μg/mL)菌索生长明显小于对照；最终的生物量以处理4（5.0 μg/mL）为最大；处理3（2.5 μg/mL）和处理4（5.0 μg/mL）蜜环菌生长状况最好。综合比较来看，以处理4（5.0 μg/mL）为蜜环菌生长的最佳浓度。该研究为后续蜜环菌的培养、菌包生产以及研发奠定了一定的基础。
- Abstract:
- Taking Armillaria mellea of Gastrodia elata Blume isolated and screened as the experimental material，the effects of different concentrations of NAA on the mycelium and rhizomorph emergence date，rhizomorph length，biomass and growth status of Armillaria mellea of Gastrodia elata Blume were investigated by using ten different concentrations of NAA added in the PDA basal culture media.The results showed that the mycelium and rhizomorph emergence dates of No.4(5.0 μg/mL) treatment were earlier than those of other treatments；the rhizomorph length of treatments from 1 to 5(0.5,1.0,2.5,5.0,10.0 μg/mL) were obviously longer than CK，while treatments from 6 to 9(25,50,100,250 μg/mL) were much worse than CK；the biomass of No.4 treatment was the highest among all the treatments；and the growth status of No.3 and 4 treatments was much better than others.On the basis of comprehensive comparison and analysis，No.4 treatment was the best one for the growth of Armillaria mellea，which laid some foundation for the cultivation，production，research and exploitation of Armillaria mellea in the future.
参考文献/References:
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备注/Memo
第一作者简介：杨文宏（1987-），男，硕士，研究方向为植物生物技术。责任作者：黄海泉(1974-)，男，博士，副教授，现主要从事生物技术等研究工作。E-mail:haiquanhuang@gmail.com.