YANG Zong-fang,MENG Qing-jie,WANG Guang-quan,et al.Study on Plant Regeneration of New Peach Variety of ‘Huangjinkui’[J].Northern Horticulture,2012,36(09):113-115.
黄桃新品种‘黄金魁’再生植株的获得
- Title:
- Study on Plant Regeneration of New Peach Variety of ‘Huangjinkui’
- 文章编号:
- 1001-0009(2012)09-0113-03
- Keywords:
- ‘Huangjinkui’ peach; tissue culture; hormone
- 分类号:
- S 662.103.6
- 文献标志码:
- A
- 摘要:
- 以黄桃新品种‘黄金魁’的带芽茎段为外植体,通过筛选不同的培养基,研究得出‘黄金魁’再生植株的最佳培养组合。结果表明:70%酒精表面消毒15 s,无菌水冲洗3次,0.1%升汞处理10 min的灭菌效果最好;芽诱导的适宜培养基为WPM +6-BA 2.0 mg/L+IBA 0.5 mg/L;幼芽形成根的最佳培养基为WPM+IBA 1.0 mg/L+NAA 0.2 mg/L,获得了‘黄金魁’桃的再生植株。该研究结果为黄桃的快速繁殖及育种奠定了基础。
- Abstract:
- Taking stem section with bud from ‘Huangjinkui’ as experiment materials,the effect of different culture medium to bud and root induced were studied to get the best culture medium for ‘Huangjinkui’.The results showed that the best culture medium for germination was WPM +6-BA 2.0 mg/L+IBA 0.5 mg/L,and the best culture medium for striking root was WPM+IBA 1.0 mg/L+NAA 0.2 mg/L.This study got the regeneration plant and the results laid a foundation for the peach rapid breeding.
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备注/Memo
第一作者简介:杨宗芳(1987-),女,山东莱州人,在读硕士,研究方向为资源植物与演化植物。E-mail:xiuzhuddd@yahoo.com.cn。
责任作者:王光全(1957-),男,本科,教授,研究方向为园艺植物种质资源。E-mail:wgq@lcu.edu.cn。
基金项目:山东省“十一五”农业科技成果转化基金资助项目(鲁科农〈2010〉79号);山东省科技攻关资助项目(2009GG10009031);聊城大学重点科研基金资助项目(X071006)。
收稿日期:2012-02-16