[1]宋利娜,康德贤,王红梅,等.辣椒三系子叶愈伤组织诱导及植株再生[J].北方园艺,2012,36(09):108-112.
　SONG Li-na,KANG De-xian,WANG Hong-mei,et al.Plant Regeneration and Callus Induction of Three Lines of Peppers via Cotyledon Culture[J].Northern Horticulture,2012,36(09):108-112.

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辣椒三系子叶愈伤组织诱导及植株再生

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷期数: 2012年09期页码: 108-112 栏目: 出版日期: 2012-09-16

Title:: Plant Regeneration and Callus Induction of Three Lines of Peppers via Cotyledon Culture

文章编号:: 1001-0009（2012）09-0109-04

作者:: 宋利娜¹; 2; 3; 康德贤³; 王红梅¹; 王益奎²; 3; 李文嘉²; 曹振木⁴; 1.广西大学农学院,广西南宁 530004；
2.广西农业科学院蔬菜研究所,广西南宁 530007;
3.广西作物遗传改良重点实验室,广西南宁 530007；
4.中国热带农业科学院热带作物品种资源研究所,海南儋州 571737

Author(s):: SONG Li-na ¹; 2; 3; KANG De-xian³; WANG Hong-mei¹; WANG Yi-kui ²; 3; LI Wen-jia²; CAO Zhen-mu⁴; 1.College of Agriculture,Guangxi University,Nanning,Guangxi 530004;
2.Institute of Vegetable Research,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007;
3.Key Laboratory of Guangxi Crop Genetic Improvement and Biotechnology,Nanning,Guangxi 530007;4.Institute of Tropical Crops Genetic Resources,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737

关键词:: 植株再生; 愈伤诱导; 激素浓度; AgNO₃

Keywords:: plant regeneration; callus induction; concentration of hormone; AgNO₃

分类号:: S 641.303.6

文献标志码:: A

摘要:: 以辣椒雄性不育系、保持系、恢复系子叶为外植体，研究了6-BA、IAA浓度组合及AgNO₃对子叶离体再生培养的影响。结果表明：三系之间在愈伤诱导和不定芽分化上均表现一定的差异，不仅最适的愈伤诱导和不定芽分化培养基不同，且分化出不定芽的数量也表现出较大差异，R-1分化出的不定芽数目明显多于A-1、B-1；AgNO₃对愈伤组织诱导没有明显的作用，但3 mg/L的AgNO₃可提高不定芽的分化率；6-BA/IAA比值为10时与比值为4时相比更有利于不定芽的分化；1/2MS+IAA 0.2 mg/L+NAA 0.1 mg/L是辣椒三系最优的生根培养基配方。

Abstract:: Taking male sterile line,maintainer line and restorer line of peppers cotyledon as explant,the factors effect of 6-BA,IAA concentration and AgNO₃on the in vitro regeneration culture of pepper cotyledon.The results showed that three lines of peppers had differences on callus and adventitious bud induction not merely the best medium of callus and adventitious bud induction but also the number of bud.The bud of R-1 easily outnumber A-1 and B-1；AgNO₃didn’t had an obvious effect on callus induction，but 3 mg/L AgNO₃ could enhance the rate of adventitious bud differentiation;The higher 6-BA/IAA ratio of ten was conductive to the differentiation of the explants of cotyledons than it was four;1/2MS+IAA 0.2 mg/L+NAA 0.1 mg/L was the optimal medium of rooting by regenerated shoots.

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备注/Memo

第一作者简介：宋利娜（1985-），女,在读硕士，研究方向为种质资源与遗传育种。E-mail:songln84@126.com。
责任作者：李文嘉（1962-），女，研究员，硕士生导师，研究方向为种质资源与遗传育种。E-mail:lwj3386@163.com。
基金项目：中央级公益性科研院所基本科研业务专项资金资助项目（PZS064）;广西自然科学基金资助项目（2011GXNSFB018054）。
收稿日期：2012-02-16

更新日期/Last Update: 2014-09-02