LI Yong-li,ZHOU Zhou,ZHEN Tao,et al.Effects of Kanamycin on Adventitious Buds Induction of Populus×euramericana cl.‘Guariento’[J].Northern Horticulture,2012,36(03):119-121.
卡那霉素对108杨不定芽诱导的影响
- Title:
- Effects of Kanamycin on Adventitious Buds Induction of Populus×euramericana cl.‘Guariento’
- 文章编号:
- 1001-0009（2012）03-0119-03
- 分类号:
- S 792.11
- 文献标志码:
- A
- 摘要:
- 以108 杨为试材,以茎段、叶柄、叶片3种外植体的高效不定芽诱导再生为基础,研究了不同梯度浓度卡那霉素(Km)对108杨不定芽诱导的影响。结果表明:茎段外植体对Km表现出较高的耐受性,叶柄和叶片外植体的敏感性高；在遗传转化过程中70 mg/L的Km作为茎段外植体的临界筛选浓度较为合适;对于叶柄和叶片外植体,选择30 mg/L的Km临界筛选浓度较为合适。
- Abstract:
- Effective adventitious buds regeneration of stems，petioles and leaves of Populus×euramericana cl.‘Guariento’ were used as foundation,influence from various Kanamycin (Km) concentrations were studied.The results showed that stem was quite tolerant to kanamycin，which proper concentration was 70 mg/L in transformation；while petioles and leaves were very sensitive and an appropriate concentration was 30 mg/L.
参考文献/References:
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备注/Memo
第一作者简介：李永丽（1978-）,女,硕士,讲师,研究方向为林木生物技术,现从事林木病理学教学与研究工作。责任作者：周洲（1978-），男，博士，副教授，现从事林木遗传育种教学与研究工作。E-mail：zhouzhouhaust@163.com。