FENG Jian,FAN Jun-gang,JIN Ruo-zhong,et al.Molecular Identification of Two Armillaria Based on IGS Sequence Analysis[J].Northern Horticulture,2012,36(03):116-118.
基于IGS序列的两株蜜环菌分子鉴定
- Title:
- Molecular Identification of Two Armillaria Based on IGS Sequence Analysis
- 文章编号:
- 1001-0009（2012）03-0116-03
- Keywords:
- Armillaria; PCR; rDNA
- 分类号:
- S 949.32
- 文献标志码:
- A
- 摘要:
- 以从猪苓[Polyporus umbellatus (Pers.) Fr.]菌核和榛蘑（Armillariella mellea）子实体中分离得到菌株bs和zm为试材，通过对其IGS区段进行分析并结合分离材料和培养菌丝的形态特征确定其分类学地位。结果表明：菌核和子实体的生境及形态学特征初步鉴定为蜜环菌；对2个菌株的分类学地位进行了分析确定，并利用引物5SA和CNL12扩增其IGS区域，进行BLAST比对分析，菌株bs和zm均为高炉蜜环菌，为辽宁省猪苓的伴生菌。
- Abstract:
- The bs and zm was isolated from sclerotia of Polyporus Umbellatus and body of Armillaria mellea respectively as test material.Through the IGS Section was analyzed and combinated the morphological characteristics of mycelium of separation material, the taxonomic position were determined. The results showed that the habitats and morphological features of sclerotia of Polyporus Umbellatus and body of Armillaria mellea，two asociated fungi were identified as Armillaria preliminarily.In order to confirm the taxonomic position of two Armillarias，PCR with 5SA and CNL12 as primers was used to amplify rDNA of two Armillarias.The results showed that both of Armillarias were Armillaria gallica
参考文献/References:
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备注/Memo
第一作者简介：冯健（1978-）,男,博士,工程师,现主要从事林木分子遗传及生物技术和土壤微生物研究工作。E-mail：fengjian-0205@163.com。