WANG Yinuo,LI Linxuan,WEI Ying,et al.Effect of Different Concentration of Hormones on Dendrobium hercoglossum Rchb.f.Tissue Culture[J].Northern Horticulture,2016,40(10):148-151.[doi:10.11937/bfyy.201610038]
不同浓度激素配比对重唇石斛组织培养的影响
- Title:
- Effect of Different Concentration of Hormones on Dendrobium hercoglossum Rchb.f.Tissue Culture
- 文献标志码:
- A
- 摘要:
- 以重唇石斛(Dendrobium hercoglossum Rchb.f.）成熟种子为外植体，MS为基本培养基，研究了植物生长调节素种类及浓度对重唇石斛继代增殖和生根的影响。结果表明：重唇石斛最佳的启动培养基为MS+6-BA 1.5 mg?L-1+NAA 0.5 mg?L-1。利于继代增殖的培养基为MS+6-BA 2.0 mg?L-1+NAA 0.4 mg?L-1+KT 0.2 mg?L-1+AC 1.0 g?L-1，最佳的生根壮苗培养基为MS+6-BA 0.5 mg?L-1+NAA 2.0 mg?L-1+AC 1.0 g?L-1，平均根长3.12 cm，生根率达到90%以上。
- Abstract:
- Dendrobium hercoglossum Rchb.f.spore was used as the explants and the MS was used as the basic culture medium，the effects of different hormone combinations on subculture，root culture were investigated.The results indicated that the best initial medium was MS+6-BA 1.5 mg?L-1+NAA 0.5 mg?L-1.The most effective medium for cluster inducing and subculture was MS+6-BA 2.0 mg?L-1+NAA 0.4 mg?L-1+KT 0.2 mg?L-1+AC 1.0 g?L-1.And the best rooting medium was MS+6-BA 0.5 mg?L-1+NAA 2.0 mg?L-1+AC 1.0 g?L-1.The average root length was 3.12 cm，and rooting ratio was above 90%.
参考文献/References:
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备注/Memo
第一作者简介：王一诺（1984-），女，广西梧州人，硕士，研究实习员，现主要从事药用植物组织培养等研究工作。E-mail：yyzwyyinuo@sina.com. 责任作者：韦坤华（1983-），女，博士，副研究员，现主要从事药用植物生物技术等研究工作。E-mail：divinekh@163.com. 基金项目：广西科学基金资助项目（桂科自0991025Z）；广西科学研究与技术开发计划资助项目（桂科合14125008-2-21）；广西医疗卫生重点科研课题资助项目（重200908）；广西科学研究与技术开发计划资助项目（桂科重1355001-3-4）。