MA Hongmei,FAN Rong.Allelopathy of Extract of Residue of Ganoderma lucidum on Hyphal Growth of Ganoderma lucidum[J].Northern Horticulture,2015,39(15):136-139.[doi:10.11937/bfyy.201515036]
灵芝菌糠提取液对灵芝菌丝体生长的化感效应
- Title:
- Allelopathy of Extract of Residue of Ganoderma lucidum on Hyphal Growth of Ganoderma lucidum
- 文献标志码:
- A
- 摘要:
- 以灵芝为试材，为探究灵芝菌糠提取液对灵芝菌丝体生长的影响，分别制备菌糠的水提取液和醇提取液，并将2种提取液分别配置成40%、60%、80%和100% 4种体积百分比浓度后加入等量的马铃薯综合培养基中培养灵芝菌丝体，记录菌丝萌发天数、菌落直径与菌丝干重，并计算其化感效应值（RI）。结果表明：菌糠不同体积浓度的水提取液均对灵芝菌丝体有抑制作用，当浓度为60%时，菌落直径与对照比最短，菌丝干重最小，其化感效应值最大，分别为-0.156和-0.289；不同体积百分比浓度的菌糠醇提取液则对菌丝的生长均起促进作用，当浓度达到60%时，菌落直径与对照相比最大，菌丝干重最大，其化感效应值最大，分别为0.311和0.483。综合比较，菌糠水提取液能抑制灵芝菌丝体的生长，但抑制作用不强；菌糠醇提取液能促进灵芝菌丝的生长，且化感作用效果比水提取更强。
- Abstract:
- Taking Ganoderma lucidum as test material，in order to study the response indices of residue of Ganoderma lucidum on ganoderma lucidum mycelium themselves，two kinds of extraction of water and anhydrous alcohol of four different gradient volume percentage concentrations of 40%,60%,80% and 100% were prepared respectively and joined in PDA media to culture Ganderma lucidum mycelia.Response indices (RI) were calculated by doing statistical analysis.The results showed that water extract of residue inhibited the hyphal growth of Ganoderma lucium.Colony diameters of different concentration treatments were shorter and the dry biomass were lighter than controls.The RIs on the diameters and biomass were -0.159 and -0.289 respectively with the 60% treatment of water extract.It was also found that anhydrous ethanol extracts with different concentration treatments of residue of Ganoderma lucidum promoted the hyphal growth of Ganoderma lucium.The RIs on the diameters and biomass were 0.311 and 0.483，respectively with the 60% treatment of anhydrous ethanol extract.It was concluded that the inhibitory effect of water extracts of residue of Ganoderma lucidum was weak，however,the promotion effect of anhydrous ethanol extracts was stronger and RIs were higher than the corresponding treatments of water extract.
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备注/Memo
第一作者简介：马红梅（1976-），女，硕士，副教授，现主要从事应用微生物及食用菌等教学与科研工作。E-mail:mahongmei612@163.com. 基金项目：海南省自然科学基金资助项目（314082）。