LI Die,WU Hao,LI Hong-yu,et al.Domestication and Cultivation of Wild Pleurotus Cystidiosus and Its Molecular Identification[J].Northern Horticulture,2015,39(04):143-146.[doi:10.11937/bfyy.201504034]
野生泡囊侧耳的鉴定及其驯化栽培
- Title:
- Domestication and Cultivation of Wild Pleurotus Cystidiosus and Its Molecular Identification
- Keywords:
- Pleurotus cystidiosus; ITS; biological characteristics; cultivation
- 文献标志码:
- A
- 摘要:
- 以采自广西大学校园内泡囊侧耳为试材，对其进行形态特征、培养特征、ITS序列（Gene Bank登录号为：KJ868811）等鉴定，并进行生物学特性研究，并利用棉籽壳、玉米芯、木糠、甘蔗渣、桑枝屑为主料进行栽培试验，以期为今后的品种使用者提供栽培方面的参考数据。结果表明：确定该菌株为泡囊侧耳Pleurotus cystidiosus；在5种供试碳源中，泡囊侧耳的菌丝利用淀粉最好；在6种供试氮源中，发现利用酵母粉最佳；泡囊侧耳的菌丝能在碳氮比10∶1～60∶1范围内生长，但以40∶1为最好；最适pH 7.0；最适生长温度范围为25.0～30.0℃，其中以27.5℃为最佳；以木糠为主料时其生物学效率最高可达到43.33%，单菇重为79.51 g/袋。
- Abstract:
- Taking Pleurotus cystidiosus as material，through morphology characteristics，culture characteristics，ITS sequence (Gene Bank accession number：KJ868811) and other identification to determine the strain of Pleurotus cystidiosus.To study its biological characteristics.Cultioation test was conducted by using cotton seed hull，corn cobs，saw dust，sugarcane bagasse which was abundant in Guangxi as substrate，to select formula of substrate suitable for Guangxi.The results showed that，in five kinds of the tested carbon sources，Pleurotus cystidiosus mycelium had better effect by using starch;In six kinds of selected nitrogen source，it was the best by using yeast powder；Pleurotus cystidiosus mycelia could grow in the carbon nitrogen ratio 10∶1—60∶1 range，but the 40∶1 was the best；the optimum growth temperature range were 25.0℃ and 30.0℃，with 27.5℃ was the best；the optimal pH 7.0.The cultivation experiment results showed that with the saw dust as main ingredient in its biological efficiency could reach 43.33%，the highest mushroom weight was 79.51 g/bag.
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备注/Memo
第一作者简介：李蝶（1989-），女，硕士，现主要从事食用菌栽培等研究工作。E-mail：385726147@qq.com.责任作者：刘斌（1966-），男，博士，教授，现主要从事食用菌及植物保护等研究工作。E-mail：liubin@gxu.edu.cn.基金项目：广西科学研究与技术开发计划资助项目(桂科攻1222012-1B)；国家食用菌产业技术体系广西创新团队建设专项资助项目。