OU Li-jun,SUN Hai-jun.Genetic Diversity Analysis of Twenty-six Species in Rose Class Based on ITS Sequences[J].Northern Horticulture,2013,37(11):94-96.
基于ITS序列的蔷薇纲二十六个物种遗传多样性分析
- Title:
- Genetic Diversity Analysis of Twenty-six Species in Rose Class Based on ITS Sequences
- 文章编号:
- 1001-0009(2013)11-0094-03
- Keywords:
- family; ITS sequences; identify; phylogenetic relationship
- 分类号:
- S 336
- 文献标志码:
- A
- 摘要:
- 利用ITS序列对蔷薇纲的桔梗科、唇形科、菊科、豆科和蔷薇科5个科的26个物种进行了遗传多样性分析。结果表明：26个物种的ITS序列全长为580~815 bp，G+C含量约为60%；基于ITS序列构建的系统树表明，科内物种优先聚类，桔梗科和唇形科优先聚类后再与菊科聚为1支，豆科和蔷薇科为另1支。可见，ITS可以将不同科的物种区分开来，但对科间关系的确定存在一定局限性。因此，ITS序列最好结合其它如形态学、结构学和其它分子标记等多种分析手段才能更加准确地分析科间亲缘关系。
- Abstract:
- The genetic analysis of 26 species including Campanulaceae,Lamiaceae,Asteraceae,Leguminosae sp.and Rosaceae were studied using ITS sequences.The results showed that the ITS lengths of 26 species were from 580 bp to 815 bp and GC content was about 60%.Clustering results based ITS sequences showed the species from the same family were firstly clustered.Five families were divided into two branches that Campanulaceae,Lamiaceae and Asteraceae were a branch and the others were a branch.The results indicated different species from different families could be identified and the identification of familial relationships had some limitations by ITS sequences.In conclusion,ITS sequences would be combined with some analytical tools such as morphology,structure and others molecular techniques to accurately analyze familial relationships.
参考文献/References:
[1]Takhtajan A.Diversity and classification of flowering plants[M].New York：Columbia University Press,1997.
[2]Thorne R F.Classification and geography of the flowering plants[J].Botanical Review,1992,58:225-348.[3]Dahlgren G.An updated angiosperm classification[J].Botanical Journal of the Linnean Society,1989,100:197-203.
[4]Cronquist A.The evolution and classification of flowering plants[M].New York：The New York Botanical Garden,1988.
[5]Melchior H A.Engler’s syllabus der pflanzenfamilien,zwolfte zuflage,II band,Angiospermen[M].Berlin：Gebruder Bomtrager,1964.
[6]吴征镒,汤彦承,路安民,等.试论木兰植物门的一级分类-一个被子植物八纲系统的新方案[J].植物分类学报,1998,36(5):385-402.
[7]葛颂,Schaal B A,洪德元,等.用核糖体DNA的ITS序列探讨裂叶沙参的系统位置—兼论ITS片段在沙参属系统学研中的价值[J].植物分类学报,1997,35(5):385-395.
[8]施苏华,杜雅青,David B,等.用cpDNA matK基因和nrDNA ITS区序列确定我国特有植物四棱草属的系统位置[J].科学通报,2003,48(11):1176-1180.
[9]杨中保,刘春霞,赵卫敬,等.灯盏花rDNA ITS序列测定及其在替代资源寻找中的意义[J].时珍国医国药,2010,21(10):2629-2632.
[10]高静.基于5.8S rDNA ITS序列的内蒙古棘豆属植物分子系统学研究[D].呼和浩特:内蒙古师范大学,2011. [11]White T J,Bruns T,Lee S,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M].In:Innes M,Gelfand D,Sninsky,etaleds.PCR protocols：a guide to methods and applications,1990:315-322.
[12]郑小艳,曹家树,滕元文.DNA序列分析在植物分子系统学研究中的应用现状-以蔷薇科为例[J].园艺学报,2009,36(12):1827-1836.
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备注/Memo
第一作者简介：欧立军（1976-），男，湖南长沙人，博士，副教授，研究方向为植物生理与分子生物学。E-mail:ou9572@126.com.
基金项目：湖南省高校科技创新团队支持计划资助项目（2010212）；湖南省“十二五”植物学重点建设学科资助项目（201142）。
收稿日期：2013-01-16