WEN Gang,LIU Hai-yan,YANG Mei.Extraction of Flavonoids from Actinidia arguta and Its Antioxidant Activity in vitro[J].Northern Horticulture,2015,39(04):140-143.[doi:10.11937/bfyy.201504033]
软枣猕猴桃黄酮的提取及体外抗氧化研究
- Title:
- Extraction of Flavonoids from Actinidia arguta and Its Antioxidant Activity in vitro
- Keywords:
- Actinidia argute; flavonoids; extraction; antioxidant
- 文献标志码:
- A
- 摘要:
- 以软枣猕猴桃为试材，采用超声辅助乙醇浸提的方法，研究了乙醇浓度、料液比、提取温度、提取时间、超声功率等因素对软枣猕猴桃黄酮提取效率的影响。对黄酮精制纯化后进一步考察软枣猕猴桃黄酮的体外抗氧化能力。结果表明：最佳提取条件是以70%(V/V)乙醇浓度为提取剂，料液比1∶8 g/mL，提取温度70℃，提取时间5 min，超声功率300 W; 软枣猕猴桃黄酮具有很好的DPPH自由基的清除能力，也具有一定的对羟基自由基和超氧阴离子自由基清除能力。
- Abstract:
- Taking Actinidia argute as test material，ultrasonic assisted extraction method was used to study the ethanol concentration，solid-liquid ratio，extraction temperature，extraction time，ultrasonic power and other factors affecting flavonoid extraction efficiency of Actinidia argute.After purification the flavonoids was used for investigated antioxidant activity in vitro.The results showed the appropriate extracted conditions were chosen as follows：ethanol concentration was 70%(V/V)，solid-liquid ratio was 1∶8 g/mL，extraction temperature was 70℃，extraction time was 5 min，and ultrasonic power was 300 W.Actinidia arguta flavonoid showed a good DPPH free radical scavenging activity，and it also had hydroxyl radicals and superoxide anion free radical scavenging ability.
参考文献/References:
[1]赵淑兰.软枣猕猴桃品种简介[J].特种经济动植物,2002（2）：35. [2]王菲,许金光,刘长江.软枣猕猴桃中功能保健成分及其在食品加工中的应用[J].食品工业科技,2010,31(8)：421-423. [3]Matich A J，Young H，Allen J M，et al.Actinidia arguta：volatile compounds in fruit and flowers[J].Phytochemistry，2003，63(3)：285-301. [4]张培成.黄酮化学[M].北京：化学工业出版社，2008：227-243. [5]王菲,栾云峰,刘长江,等.响应面分析法优化微波辅助提取软枣猕猴桃黄酮[J].食品研究与开发,2010，31(9)：6-11. [6]何书美,刘敬兰.茶叶中总黄酮含量测定方法的研究[J].分析化学研究简报,2007,35(9)：1365-1368. [7]Yen G C，Chen H Y.Antioxidant activity of various tea extracts in reaction to their antimutagenicity [J].Journal of Agricultural and Food Chemistry，1995，43(1)：27-32. [8]Winterbourn C C，Sutton H C.Hydroxyl radical production from hydrogen peroxide and enzymatically generated paraquat radicals：catalytic requirements and oxygen dependence [J].Archives of Biochemistry and Biophysics，1984，235(1)：116-126. [9]Shimada K，Fujikawa K，Yahara K T.Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion[J].Journal of Agricultural and Food Chemistry，1992，40(6)：945-948.
相似文献/References:
[1]曾照旭,朴一龙,金东淳,等.1-甲基环丙烯处理对软枣猕猴桃果实软化的影响[J].北方园艺,2014,38(01):123.
ZENG Zhao-xu,PIAO Yi-long,JIN Dong-chun,et al.Effects of 1-MCP Treatment on Softening of Actinidia arguta Fruit[J].Northern Horticulture,2014,38(04):123.
[2]姜少娟,刘晓莉.生姜黄酮超声提取及其抑菌活性研究[J].北方园艺,2014,38(04):120.
JIANG Shao-juan,LIU Xiao-li.Study on Ultrasonic Extraction of Flavonoids From Ginger and Antibacterial Activity[J].Northern Horticulture,2014,38(04):120.
[3]李红娟,樊金拴,郑涛,等.响应面法优化核桃青皮黄酮的超声提取工艺研究[J].北方园艺,2013,37(14):132.
LI Hong-juan,FAN Jin-shuan,ZHENG Tao,et al.Response Surface Methodology for the Optimization of Extraction Technology of Total Flavonoids from Walnut Green Husk[J].Northern Horticulture,2013,37(04):132.
[4]李 光,余 霜,邓 银,等.金荞麦叶总黄酮含量测定方法研究[J].北方园艺,2013,37(17):154.
LI Guang,YU Shuang,DENG Yin,et al.Study on the Determination Method of Flavonoids in Fagopyrum cymosum Complex Leaves[J].Northern Horticulture,2013,37(04):154.
[5]焦 镭,柴梦颖,石明生,等.碱性条件下山楂叶总黄酮的提取工艺研究[J].北方园艺,2014,38(08):128.
JIAO Lei,CHAI Meng-ying,SHI Ming-sheng,et al.Study on the Extraction Technology of Total Flavonoids From Hawthorn Leaf in Alkaline Conditions[J].Northern Horticulture,2014,38(04):128.
[6]王丽霞,刘坤.超声波辅助提取玉米须黄酮工艺[J].北方园艺,2014,38(06):137.
WANG Li-xia,LIU Kun.Extraction Technology on Flavonoids From Corn Silk by Ultrasonic-assisted[J].Northern Horticulture,2014,38(04):137.
[7]王忠忠,马茜,慕金凤,等.宁夏枸杞果实发育过程中黄酮类化合物含量的变化研究[J].北方园艺,2013,37(11):152.
WANG Zhong-zhong,MA Qian,MU Jin-feng,et al.Changes of Flavonoid Compounds Content During the Fruit Development of Lycium barbarum L.[J].Northern Horticulture,2013,37(04):152.
[8]胡喜巧,陈红芝,孟丽.采收时期对红花苗菜产量及营养成分的影响[J].北方园艺,2013,37(12):165.
HU Xi-qiao,CHEN Hong-zhi,MENG Li.Influence of Harvesting Time on the Yield and Nutritional Composition of Safflowers Vegetable[J].Northern Horticulture,2013,37(04):165.
[9]李光.,余霜.,陈庆富.正交设计在红薯叶黄酮提取中的应用[J].北方园艺,2012,36(01):41.
LI Guang.,YU Shuang.,CHEN Qing-fu.The Application of Extracting Flavonoids from Sweet Potato Leaves by Orthogonal Method[J].Northern Horticulture,2012,36(04):41.
[10]王宁,杨玉红,康宗利,等.软枣猕猴桃植株的过氧化物酶同工酶酶谱差异研究[J].北方园艺,2012,36(04):120.
WANG Ning,YANG Yu-hong,KANG Zong-li,et al.Study on the Differences of Peroxidase Isozyme Electrophoretogram in Plants of Actinidia arguta[J].Northern Horticulture,2012,36(04):120.
备注/Memo
第一作者简介：温钢（1976-），男，硕士，讲师，现主要从事多糖的提取与活性等研究工作。E-mail：315554062@qq.com.