QIAN Xueting,CHEN Wenqiang,DENG Baiwan,et al.Analysis of the Genetic Diversity of Auricularia auricular Strains From Qinba Mountains Based on ITS Sequence[J].Northern Horticulture,2016,40(12):147-151.[doi:10.11937/bfyy.201612037]
基于ITS序列分析秦巴山区黑木耳菌株的遗传多样性
- Title:
- Analysis of the Genetic Diversity of Auricularia auricular Strains From Qinba Mountains Based on ITS Sequence
- 文献标志码:
- A
- 摘要:
- 通过黑木耳菌株的ITS序列分析，对秦巴山区44个黑木耳菌株进行遗传多样性研究。采用CTAB法提取黑木耳菌株基因组DNA，进行PCR扩增，对扩增产物测序，并通过软件ClustalX 1.83和MEGA 5.0进行序列比对以及系统发育树的构建。结果表明：秦巴山区黑木耳菌株遗传多样性较丰富，部分菌株之间同源性较高，甚至可能为同一品种，当相似系数为0.002时，聚类为5个分支，其中耳268与其它菌株亲缘关系较远，为今后秦巴山区黑木耳菌株的遗传多样性及优良品种的遗传育种奠定了理论基础。
- Abstract:
- Genetic diversity among different strains were researched by analyzing the morphological characters and ITS sequence of 44 Auricularia auricular strains from Qinba Mountains.Using CTAB method to extract genomic DNA,amplifying and sequencing PCR products.The software CluatalX and MEGA 5.0 were applied to sequence alignment and phylogeny analysis.The results showed that plentiful genetic diversity among different strains was found,while the homology of parts of Auricularia auricular strains was high.Based on the similarity coefficient about 0.002,the strains were separated into five groups.The genetic relationship between Auricularia auricula-judae 268 and others were quite far.
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备注/Memo
第一作者简介：钱雪婷（1990-），女，陕西汉中人，硕士研究生，研究方向为微生物资源利用开发。E-mail:qianxueting163@163.com.责任作者：陈文强（1956-），男，陕西汉中人，教授，现主要从事微生物资源的保护与利用等研究工作。E-mail:wenqiangc@126.com.基金项目：陕西省科学技术研究发展计划资助项目（2014K01-16-03）；陕西省科技统筹创新工程计划资助项目（2012HBGC-20）。