[1]刘主,蔡爱群,刘静华,等.基于ITS区序列的疑似野生“南华”草菇菌株的分子鉴定[J].北方园艺,2016,40(01):126-130.[doi:10.11937/bfyy.201601033]
　LIU Zhu,CAI Aiqun,LIU Jinghua,et al.Molecular Identification of Suspected Wild ‘Nanhua’ Volvariella volvacea Strain Based on Internal Transcribed Spacer Sequence[J].Northern Horticulture,2016,40(01):126-130.[doi:10.11937/bfyy.201601033]
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基于ITS区序列的疑似野生“南华”草菇菌株的分子鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷 期数: 2016年01 页码: 126-130 栏目: 出版日期: 2016-01-15
Title:
Molecular Identification of Suspected Wild ‘Nanhua’ Volvariella volvacea Strain Based on Internal Transcribed Spacer Sequence
作者:
刘主1; 蔡爱群1; 刘静华1; 何智丹2; 孟晓东1
（1.韶关学院 英东生命科学学院，广东 韶关 512005；华南农业大学 生命科学学院，广东 广州 510640）
Author(s):
LIU Zhu1; CAI Aiqun1; LIU Jinghua1; HE Zhidan2; MENG Xiaodong1
(1.Yindong College of Life Sciences，Shaoguan University，Shaoguan，Guangdong 512005；2.College of Life Sciences，South China Agricultural University，Guangzhou，Guangdong 510640)
关键词:
草菇; 野生菌株; ITS; BLAST; 分子鉴定
Keywords:
Volvariella volvacea; wild strain; ITS sequence; BLAST; molecular identification
DOI:
10.11937/bfyy.201601033
文献标志码:
A
摘要:
以在广东省韶关市“南华寺”周边山岭采得的1株疑似野生“南华”草菇菌株VNH为试材，采用ITS序列克隆和分析，并结合传统形态学研究对其进行分子鉴定。结果表明：菌株VNH为野生“南华”草菇；为进一步研究该野生菌株的生物学特性及驯化栽培研究提供了重要依据；同时，对丰富草菇菌种资源，促进韶关文化和旅游发展具有积极意义。
Abstract:
A suspected wild ‘Nanhua’ Volvariella volvacea was picked beside the mountains next to Nanhua temple in Shaoguan city of Guangdong Province.The pure culture was obtained by tissue isolation and was named strain VNH.The strain was identified by molecular identification with ITS sequence cloning and analyzing and combine with the traditional morphological.The results showed that,VHN strain was wild ‘Nanhua’ V.volvacea.It provided an important basis on the studies of biological characteristics and domestication cultivation for the wild strain.Meanwhile，it had a positive significance to enrich V.volvacea species resources and to promote the development of Shaoguan culture and tourism.

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备注/Memo

第一作者简介：刘主（1977-），男，湖南宁远人，博士，副教授，现主要从事食用菌和分子生物学的教学与科研等工作。E-mail：liuzhu77@126.com.基金项目：韶关市科技计划资助项目（2013CX/K）；韶关学院大学生创新创业训练计划资助项目（Sycxcy2014-028）。

更新日期/Last Update: 2016-03-02