ZHU Yunhao,DU Zhenhui,DONG Chengming,et al.Diversity of Endophytic Fungi From Tubers of Rehmannia glutinosa Libosch[J].Northern Horticulture,2016,40(03):145-149.[doi:10.11937/bfyy.201603041]
地黄块根内生真菌的多样性研究
- Title:
- Diversity of Endophytic Fungi From Tubers of Rehmannia glutinosa Libosch
- Keywords:
- Rehmannia glutinosa Libosch; endophytic fungi; ITS
- 文献标志码:
- A
- 摘要:
- 以产自河南温县的地黄块根为试材，采用平板分离法分离地黄内生真菌，通过形态学特征和核糖体转录间隔区（ITS）序列分析，对分离出的真菌进行分类鉴定；以内生真菌的分离率（IR）、定殖率（CR）、相对频率（RF）、多样性指数（H）及均匀度指数为指标研究地黄块根内生真菌的菌群组成及其多样性。结果表明：从地黄块茎中分离得到 81 株内生真菌纯化培养物，经鉴定28株为镰孢霉属 Fusarium；19株为青霉属Penicillium；15 株为茎点霉属Phoma；12株为链格孢属 Alternaria；其它 6 株分别为赤霉属Gibberella、曲霉属Aspergillu，土赤壳属Ilyonectria、毛壳属Chaetomium。镰孢霉属、青霉属、茎点霉属、链格孢属为地黄块根内生真菌优势菌群，地黄内生真菌菌群组成具有一定程度的多样性。
- Abstract:
- Taking tubers of R.glutinosa from Wen county in Henan Province as materials,the plate cultivation methods were used to isolate the endophytic fungi.The endophytic fungi were identified based on morphology and ITSrDNA sequences.The results were analyzed by the indexes such as isolation rate (IR),colonizationrate(CR),relative frequency(RF)，Shannon-Wiener biodiversity index (H).The results showed that 28 strains were classified as Fusarium,19 strains were slassified as Penicillium,15 strains were classified as Phoma，12 strains were classified as Alternaria and the other 6 strains were classified as Gibberella，Aspergillu，Ilyonectria、Chaetomium.Among the isolates，Fusarium,Penicillium,Phoma,Alternaria were the dominant genera．The composition of endophytic fungi community had a certain diversity in the tubers of R.glutinosa.
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备注/Memo
第一作者简介：朱畇昊（1986-），男，博士，讲师，现主要从事药用植物分子生物学等研究工作。E-mail:guxinhan123@163.com.责任作者：董诚明(1963-)，男，本科，教授，现主要从事药用植物规范化种植等研究工作。E-mail:dcm371@sohu.corn.基金项目：国家“十二五”科技支撑计划资助项目(2011BAI06802)。