LEI Xiao-fei,YANG Xue-ling.Optimization of Culture Conditions in vitro Dendranthema indicum var.aromaticum Buds of Orthogonal Test[J].Northern Horticulture,2014,38(16):105-108.
正交实验优化“神农”香菊顶芽的离体培养条件
- Title:
- Optimization of Culture Conditions in vitro Dendranthema indicum var.aromaticum Buds of Orthogonal Test
- 文章编号:
- 1001-0009(2014)16-0105-04
- Keywords:
- Dendranthema indicum var.aromaticum; tissue culture; rapid propagation technique; orthogonal test
- 分类号:
- S682.1+1
- 文献标志码:
- A
- 摘要:
- 以湖北神农架地区采集的“神农”香菊顶芽为外植体，研究了不同消毒剂种类及方法的灭菌效果，通过芽增殖培养基和生根培养基的激素配比试验，筛选出“神农”香菊试管苗快速繁殖的最佳培养基组成。结果表明：“神农”香菊顶芽的最佳消毒方案为75% Alcohol 30 s+0.1% HgCl2 2 min+2% NaClO3 8 min；顶芽最适增殖培养基为MS+0.7 mg/L 6-BA+0.5 mg/L KT+0.3 mg/L NAA，生根的最适培养基为1/2 MS+0.5 mg/L NAA。
- Abstract:
- Taking buds of Dendranthema indicum var.aromaticum introduced from Shennongjia of Hubei as the explants,the sterilizing effect was studied through different disinfectors and methods,by hormone combination test on sprout medium and rooting medium,rooting hormone ratio were studied to selected the best medium for rapid propagation of Dendranthema indicum var.aromaticum were studied.The results showed that the suitable disinfectant was 75% Alcohol 30 s+0.1% HgCl2 2 min+2% NaClO3 8 min；the suitable sprout medium was MS+0.7 mg/L 6-BA+0.5 mg/L KT+0.3 mg/L NAA;the suitable rooting medium was 1/2 MS+0.5 mg/L NAA.
参考文献/References:
［1］谢超,黄龙,肜霖,等.正交试验优选“神农”香菊挥发油提取条件［J］.湖北农业科学,2011,50(21):4451-4453.
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备注/Memo
第一作者简介：雷霄飞（1979-），男，硕士，讲师，研究方向为生物化学与分子生物学。E-mail：48729155@qq.com.