[1]张丽珍,杨冬业,刘杰,等.超富集植物商陆多倍体诱导研究[J].北方园艺,2014,38(16):109-111.
　ZHANG Li-zhen,YANG Dong-ye,LIU Jie,et al.Study on Polyploid Induction of Hyperaccumulator Plant—Phytolacca acinosa Roxb.[J].Northern Horticulture,2014,38(16):109-111.

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超富集植物商陆多倍体诱导研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷期数: 2014年16期页码: 109-111 栏目: 出版日期: 2014-08-30

Title:: Study on Polyploid Induction of Hyperaccumulator Plant—Phytolacca acinosa Roxb.

文章编号:: 1001-0009(2014)16-0109-03

作者:: 张丽珍¹; 杨冬业²; 刘杰³; 曾惠玲¹; 1．桂林师范高等专科学校化学与药学系，广西桂林 541001；
2．桂林医学院,广西桂林 541001；
3．桂林理工大学广西矿冶与环境科学实验中心，广西桂林 541001

Author(s):: ZHANG Li-zhen¹; YANG Dong-ye²; LIU Jie³; ZENG Hui-ling¹; 1.Department of Chemistry and Pharmacy,Guilin Normal College,Guilin,Guangxi 541001;
2.Guilin Medical College,Guilin,Guangxi 541001;
3.Guangxi Scientific Experiment Center of Mining,Metallurgy and Environment,Guilin University of Technology,Guilin,Guangxi 541004

关键词:: 商陆; 多倍

Keywords:: Phytolacca acinosa Roxb.; polyploid; induced by colchicine

分类号:: Q813

文献标志码:: A

摘要:: 以商陆为试材，对商陆幼苗进行秋水仙素诱导多倍体试验。结果表明：0.2%秋水仙素诱变48 h效果最好，与对照相比，变异株茎粗壮，叶宽、叶厚较对照分别增加40.15%和33.25%；气孔纵横径较对照分别增加30.25%和60.82%，气孔密度增加150%；压片法对变异植株和对照植株的根尖有丝分裂分别进行染色体鉴定，证明变异株为多倍体。

Abstract:: Taking Phytolacca acinosa Roxb.as material,Phytolacca acinosa Roxb.seedlings were treated with different concentrations of colchicines solution，polyploid induction test was conducted.The results showed that the best treatment was using 0.2% colchicines solution and 48 hours.Compared with the control,the stems of variant plants became more dwarf and stronger,leaf width was increased by 40.15% and leaf thickness was increased by 33.25%.Length and width of stomata was increased by 30.25% and 60.82% respectively,the density of stomata was increased by 150%;the variant plants were polyploid by tabletting method of mutant plants and control of the plant root mitotic chromosome identification respectively.

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备注/Memo

第一作者简介：张丽珍（1979-），女，广西贵港人，硕士研究生，副教授，现主要从事生物工程等研究工作。E-mail：xiaozhang446@163.com.

基金项目：国家自然科学基金资助项目(41001186)。

更新日期/Last Update: 2014-09-26