WEI Jian,WANG Wen-hui,WANG Li-hao,et al.Comparison of Tissue Culture and Oil Content on Different Varieties of Safflower[J].Northern Horticulture,2013,37(19):106-109.
不同红花品种的组织培养及油脂含量的比较
- Title:
- Comparison of Tissue Culture and Oil Content on Different Varieties of Safflower
- 文章编号:
- 1001-0009(2013)19-0106-04
- Keywords:
- safflower; tissue culture; fat content
- 分类号:
- S 503.53
- 文献标志码:
- A
- 摘要:
- 以5个不同品种的红花为试材，研究比较了其种子萌发率，组织培养过程中的污染率、分化率和生根率，以及籽粒含油率和可溶性蛋白质含量等。结果表明：“吉红早熟”的种子萌发率高，分化率相对较高，尤其生根率较其它几个品种均较高，所以此品种适合作为红花组织培养的基因型，另外，“吉红早熟”品种的种子含油量较高，适合开发成为红花油类产品。
- Abstract:
- Taking 5 different varieties of safflower as materials，the seeds germination rate，the contamination rate，differentiation rate and rooting rate in the process of tissue culture and oil content and protein content in seed were studied and compared.The results showed that ‘Jihongzaoshu’ safflower had high seed germination rate and differentiation rate，especially the rooting rate compared with several other varieties was higher，so this breed was suitable as tissue culture genotype.‘Jihongzaoshu’ safflower seed had high oil content，so it was suitable for the development of a number of safflower oil products.
参考文献/References:
[1]Li D，Mundel H H.Safflower，Carthamus tinctorius?L.promoting the conservation and use of underutilized and neglected crops[J].Gatersleben/IPCRI，Rome,1996,5(3):119-132. [2]杨晶,李海燕,付红歧,等.红花遗传转化受体系统的建立及转酸性成纤维细胞生长因子aFGF的初步研究[J].中国中药杂志，2010,35(4):411-414. [3]王兆木.红花[M].北京:中国中医药出版社,2001:148-180. [4]花方信孝,培养红花细胞产生红花色素[J].廖系晗，译.四川中草药研究,1992,3(6):33-34. [5]甘烦远,郑光植.植醇与寡糖对红花悬浮培养细胞的生长及α-生育酚积累的效应[J].植物生理学报,1990,16(4):361-366. [6]王镨,邵鸥,岑沛霖.红花细胞培养条件的优化及其胞内产物生育酚的积累[J].生物工程学报,1999,15(4):444-449. [7]周平,郑光植.红花细胞克隆的平板培养[J].植物学报,1989,31(7):505-511. [8]周平,郑光植.红花单细胞克隆的建立[J].植物学报,1989,31(9):661-667. [9]甘烦远,郑光植.红花愈伤组织诱导、生长及其α-生育酚的产生[J].云南植物研究,1991,13(2):189-195. [10]甘烦远,郑光植.各种因素对红花培养物中细胞生长及α-生育酚含量的影响[J].植物学报,1991,33(7):516-522. [11]甘烦远,郑光植.红花细胞培养中高产α-生育酚克隆系的筛选[J].云南植物研究,1992,14(3):289-294. [12]靳占忠,候占铭,韩碧文.红花的愈伤组织诱导及其与过氧化物酶和酯酶同工酶的关系[J].植物生理学通讯,1989(8):15-18. [13]侯占铭,韩碧文.红花组织培养中细胞分化的超微结构研究[J].植物学报,1994,36(增刊):61-66. [14]George L，Rao P S.In vitro multiplication of safflower (Carthamus tinctorius?L.) through tissue vulture[J].Biol Sci,1982,48(6):791-794. [15]刘仁建.红花种子醇溶蛋白及其含油率和脂肪酸分析[D].雅安:四川农业大学,2006.
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备注/Memo
第一作者简介：魏健（1980-），男，博士，副教授，研究方向为药用植物组织培养。E-mail:148050459@qq.com.