[1]高珊,林梅,崔建国,等.蒙古栎促萌茎段的离体培养研究[J].北方园艺,2013,37(19):102-105.
　GAO Shan,LIN Mei,CUI Jian-guo,et al.Study on in vitro Culture of Forced Shoots of Quercus mongolica[J].Northern Horticulture,2013,37(19):102-105.
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蒙古栎促萌茎段的离体培养研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年19期 页码: 102-105 栏目: 出版日期: 2013-10-15
Title:
Study on in vitro Culture of Forced Shoots of Quercus mongolica
文章编号:
1001-0009(2013)19-0102-04
作者:
高珊1; 林梅1; 崔建国1; 林强2; 刘静宇3
1.沈阳农业大学 林学院，辽宁 沈阳 110866；
2.本溪市林业技术推广培训中心，辽宁 本溪 117022；
3.本溪市林业科学研究所，辽宁 本溪 117022
Author(s):
GAO Shan1; LIN Mei1; CUI Jian-guo1; LIN Qiang2; LIU Jing-yu3
1.College of Forestry，Shenyang Agricultural University，Shenyang，Liaoning 110866；
2.Forestry Technology Extension and Training Center of Benxi City，Benxi，Liaoning 117022；
3.Forestry Science Institute of Benxi City，Benxi，Liaoning 117022
关键词:
蒙古栎; 促萌; 组织培养; 植株再生
Keywords:
Quercus mongolica; forced shoot; tissue culture; plant regeneration
分类号:
S 792.186
文献标志码:
A
摘要:
以蒙古栎促萌枝条的幼嫩茎段为外植体，研究了不同种类的基本培养基、不同浓度的外源激素及其配比对蒙古栎器官发生及植株再生的影响。结果表明：蒙古栎促萌枝条的幼嫩茎段可通过器官发生途径获得再生植株，最佳初代培养基为WPM+6-BA 0.5 mg/L+2,4-D 0.5 mg/L，诱导率为76.7%；最佳不定芽增殖培养基WPM+6-BA 2.0 mg/L+NAA 0.5 mg/L，增殖系数为3.5；最佳生根培养基为1/2MS+NAA 0.5 mg/L+IBA 0.1 mg/L，生根率达76.7%。
Abstract:
Taking the forced shoots of Quercus mongolica as a source of initial explants，the effects of different types of media and different concentrations of plant growth regulators and their ratios on organogenesis and plantlet regeneration of Quercus mongolica were studied.The results showed that regenerated plants could be obtained from forced shoots of Quercus mongolica via organogenesis.The best initial medium was WPM+6-BA 0.5 mg/L+2,4-D 0.5 mg/L,the induction rate was 76.7%.The best adventitious bud proliferation medium was WPM+6-BA 2.0 mg/L+NAA 0.5 mg/L and the highest proliferation factor was 3.5.The best rooting medium was 1/2MS+NAA 0.5 mg/L+IBA 0.1 mg/L and the rooting rate was up to 76.7%.

参考文献/References:

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相似文献/References:

[1]王 晓 春.蒙古栎大树移植技术研究[J].北方园艺,2010,34(10):0.[doi:10.11937/bfyy.2010010048]

备注/Memo

第一作者简介：高珊（1987-），女，满族，辽宁本溪人，硕士研究生，现主要从事林业生物技术研究工作。E-mail:gaoshan2010@126.com.

责任作者：崔建国（1969-），男，博士，教授，研究方向为林木遗传育种和生物技术。E-mail:cjgsau@yahoo.com.cn.

更新日期/Last Update: 2014-08-26