FUTing,LIBo-lin.PreliminaryStudyontheTechniqueofTissueCultureandRapidPropagationon Fast-growingGuangxiChineseFir[J].Northern Horticulture,2013,37(16):111-113.
速生广西良种杉木组培快繁技术初步研究
- Title:
- PreliminaryStudyontheTechniqueofTissueCultureandRapidPropagationon Fast-growingGuangxiChineseFir
- 文章编号:
- 1001-0009（2013）16-0111-03
- Keywords:
- Chinesefir; tissueculture; multiplicationcoefficient
- 分类号:
- S791.27
- 文献标志码:
- A
- 摘要:
- 以杉木优良无性系树干基部萌条为试材，研究了培养基类型、活性炭和不同激素组合对杉木苗生长的影响。结果表明：杉木组织培养的适宜生长条件为MS+1.0mg/L6-BA+0.3mg/LNAA+3%蔗糖，45d增殖系数可达到3.05；同样的配方，不同的材料增殖系数不同；活性炭可以降低玻璃化，但作用时间过长会使苗老化。
- Abstract:
- Takingshootsofexcellenttreetrunkfromchinesefirasmaterials，theeffectsof4factors，medium,activatedcarbon,6-BAandNAA,onthebudpropagationofitwerestudied.TheresultsshowedthatMSmediumsupplementedwith6-BA1.0mg/LandNAA0.3mg/Lwassuitableforitsmultiplication，themultiplicationcoefficientcouldreach3.05after45days；thesameformula，theproliferationcoefficientofdifferentmaterial；activatedcarboncouldreducethevitrification，buttimewastoolongwillcauseageing.
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备注/Memo
第一作者简介：付婷（1986-），女，江西进贤人，硕士研究生，研究方向为植物资源研究与开发。E-mail：futing8681@126.com.
责任作者：李伯林（1964-），男，广西柳州人，博士，副教授，研究方向为细胞培养与育种。E-mail：Libolin-2006@163.com.
收稿日期：2013-04-10