XUE Yin-fang,ZHAO Da-qiu,ZHOU Chun-hua,et al.Research Advances of Herbaceous Peony Tissue Culture[J].Northern Horticulture,2012,36(04):167-170.
芍药组织培养的研究进展
- Title:
- Research Advances of Herbaceous Peony Tissue Culture
- 文章编号:
- 1001-0009(2012)04-0167-04
- Keywords:
- herbaceous peony; tissue culture; explant; regeneration pathway
- 分类号:
- S 682.1+2
- 文献标志码:
- A
- 摘要:
- 利用组织培养进行繁育是芍药商品化和产业化发展的必然趋势。现从外植体选择与处理、再生体系途径(愈伤组织途径、丛生芽途径、体细胞胚途径)、目前组培中存在的问题(主要是褐化和玻璃化)等方面,综述了芍药组织培养研究现状,并对今后的发展进行了展望,旨在为芍药的生产提供重要信息。
- Abstract:
- Traditional propagation methods of herbaceous peony have many problems,while propagation seedlings using tissue culture methods is the inevitable trend during development of commercialization and industrialization of herbaceous peony.The research situations of the tissue culture of herbaceous peony were reviewed from three aspects,selection and treatment of explants,regeneration pathways (callus,bud,and somatic embryo),the problems existed in tissue culture (browning and glassing) and so on.The future development of tissue culture in herbaceous peony was prospected in the final.And hoped these illustrations could provide some important information for for herbaceous peony production.
参考文献/References:
[1]郭先锋.中国芍药分类学研究进展[J].北京林业大学学报,2002,24(3):99-102.
[2]秦魁杰.芍药[M].北京:中国林业出版社,2004.
[3]高莉萍.月季品种‘萨蔓莎’植株再生体系的建立和根癌农杆菌介导的遗传转化研究[D].武汉:华中农业大学,2004.
[4]马慧,张立军,阮燕烨,等.植物组织培养技术的现状及发展趋势[J].安徽农业科学,2007,35(6):1602-1604.
[5]董少鸣.愈伤组织培养实验中几种外植体的选择[J].承德民族师范高等专科学校学报,2007,27(5):62.
[6]张越阳,魏军亚,李开绵.芒果组织培养研究进展[J].热带作物学报,2010,31(2):325-331.
[7]黄凤兰,孟凡娟,牛红云,等.芍药遗传转化再生体系的建立[J].东北农业大学学报,2009,40(6):50-57. [8]潘瞳,吴红娟,于晓南.芍药丛生芽及愈伤组织诱导方法初探[C].中国观赏园艺研究进展,2009:245-249. [9]王吉凤,李青,孟会.5个芍药品种愈伤组织诱导及分化研究[J].北京林业大学学报,2010,32(3):213-216. [10]金飚,何小弟,吴建华,等.芍药离体培养初步研究[J].江苏农业科学,2005(4):69-71.
[11]郭风云.芍药组织培养技术的研究[D].北京:北京林业大学,2001.
[12]朱广廉.植物组织培养中的外植体灭菌[J].植物生理学通讯,1996,32(6):444-449.
[13]张庆瑞,孙建洲,任凝辉,等.芍药组织培养技术研究[J].河南农业科学,2006(4):88-90.
[14]张庆瑞,杨秋生,李永华.不同植物生长调节物质对芍药离体培养的影响[J].河南农业大学学报,2007,41(1):25-28.
[15]赵蓉.芍药愈伤组织诱导及增殖研究[D].北京:北京林业大学,2009.
[16]赵明,张松荣,何小弟,等.芍药愈伤组织诱导及遗传转化技术的初步研究[J].林业实用技术,2009(1):10-12.
[17]王吉凤.芍药组织培养研究[D].北京:北京林业大学,2009.
[18]胡映泉,冯海华,时宝凌.芍药不定芽的诱导技术[J].山西林业科技,2003,增(S1):23-24,33.
[19]吴红娟,于晓南.国内外芍药组织培养研究进展[J].安徽农业科学,2010,38(2):617-619.
[20]张庆瑞.芍药组织培养技术的初步研究[D].郑州:河南农业大学,2006.
[21]Takashi A H,Michiko A,Takehito K.In vitro propagation of herbaceous peony (Paeonia lactiflora Pal1.) by a longitudinal shoot-split method [J].Plant Cell Reports,1989(8):243-246.
[22]Gabryszewska E.The influence of cytokinins,thidiazuron,paelobutrazol and red light on shoot proliferation of herbaceous paeony cv.Jadwiga in vitro[J].Journal of Fruit and Ornamental PIant Research,1998,6(3/4):157-169.
[23]兰海英.芍药的胚培养及芍药苷含量的研究[D].呼和浩特:内蒙古大学,2003.
[24]王傲雪,李景富.植物体细胞胚状体的诱导研究及应用[J].黑龙江农业科学,1999(2):39-41.
[25]Brukhin V B,Batygina T B.Embryo culture and somatic embryogenes is in culture of anoma [J].Phytomorphology,1994,44(3/4):151-157.
[26]Kim Y S,Lee B K.Effect of plant growth regulators and culture temperature on embryo culture of Paeonia albiflora[J].Jonmal of the Korean Society for Horticultural Science,1995,36(2):255-262.
[27]Kim Y S,Lee B K.Somatic embryogenesis and plant regeneration in cotyledon culture of Paeonia albiflora[J].Journal of the Korean Society for Horticultural Science,1996,37(6):827-830.
[28]Kim H M,Shin J H,Sohn J K.Cryopreservation of somatic embryos of the herbaceous peony (Paeonia lactiflora Pall.) by air drying [J].Cryobiology,2006,53:69-74.
[29]Kunneman B P A M,Albers M R J.Tissue culture of peony is not yet producing plants [J].Bloembollencultuur,1989,100(23):16-17.
[30]Seyring M.Loss rate is still too high [J].TASPO Gartenbaumazin,2000,10(2):30-31.
[31]王瑶,岳桦.芍药属植物组织培养中褐化问题的研究进展[J].黑龙江农业科学,2009(2):159-160.
[32]安佰义,赵飞.牡丹不同类型总酚含量与PPO活性研究[J].北华大学学报(自然科学版),2005,6(2):169-172.
[33]何松林,陈笑蕾,陈莉,等.牡丹叶柄离体培养中褐化防止的初步研究[J].河南科学,2005,23(1):47-50. [34]张俊琦,罗晓芳.牡丹组织培养中褐化的发生原因与防止方法的研究[J].沈阳农业大学学报,2006,37(5):720-724.
[35]刘庆昌,吴国良.植物细胞组织培养[M].北京:中国农业大学出版社,2003:44-45.
[36]贾海慧.影响樱桃试管苗玻璃化的因素与克服措施[J].山东林业科技,2010(4):72-73.
[37]任东植,李峰,曲云琴.影响枣组培苗玻璃化的几个因素及其防治[J].植物生理学通讯,2000(1):21-23. [38]朱根发.花卉组织培养-培养基及其配制[J].花卉,2002(5):6.
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备注/Memo
第一作者简介:薛银芳(1986-),女,硕士,现主要从事园艺植物栽培与生理研究工作。
责任作者:陶俊(1966-),男,博士,教授,博士生导师,现主要从事园艺植物栽培生理与分子生物学等研究工作。
基金项目:江苏省科技支撑计划(农业)资助项目(BE2009318);江苏省科技基础设施建设计划(科技公共服务平台)资助项目(BM2010590)。
收稿日期:2011-12-14