LIU Hai-ping.The Application Status of Virus Induced Gene Silencing (VIGS) in Plant[J].Northern Horticulture,2012,36(04):171-173.
病毒诱导的基因沉默在植物中的应用现状
- Title:
- The Application Status of Virus Induced Gene Silencing (VIGS) in Plant
- 文章编号:
- 1001-0009(2012)04-0171-03
- Keywords:
- virus induced gene silencing; vectors; small interfere RNA; microRNA
- 分类号:
- S 603.6
- 文献标志码:
- A
- 摘要:
- 传统的病毒诱导的基因沉默（Virus induced gene silencing，VIGS）是一种小干扰RNA（Small interfering RNAs，siRNA）介导的RNA沉默技术（SIR VIGS），作为一种有效的反向遗传学技术广泛的用于植物基因组功能的鉴定。另外，VIGS技术开始用来研究植物内源miRNA（microRNA）的功能，这为miRNA的功能研究提供了快速有效的方法。随着基因沉默技术的应用和改进，将会有越来越多的植物基因组功能得到快速有效的验证。
- Abstract:
- Small interfere RNA plays the main role in traditional virus induced gene silencing which is a reverse genetics technology and has been widely used in plants to study gene function.Besides，VIGS also provides a rapid and effective method in researching function of miRNA.As the development of VIGS，more and more gene function will be validated in plant in the future.
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备注/Memo
作者简介：刘海萍（1988-），女，在读硕士，研究方向为食品生物技术。
基金项目：国家自然科学基金资助项目（31000925）。
收稿日期：2011-12-08