[1]姚鹏强,曲雪莲,程世平.野生猕猴桃同源四倍体诱导[J].北方园艺,2020,44(15):42-47.[doi:10.11937/bfyy.20194173]
　YAO Pengqiang,QU Xuelian,CHENG Shiping.Induction of Autotetraploid for Wild Kiwifruit[J].Northern Horticulture,2020,44(15):42-47.[doi:10.11937/bfyy.20194173]
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野生猕猴桃同源四倍体诱导

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年15 页码: 42-47 栏目: 出版日期: 2020-08-15
Title:
Induction of Autotetraploid for Wild Kiwifruit
作者:
姚鹏强; 曲雪莲; 程世平
（平顶山学院 化学与环境工程学院，河南 平顶山 467000）
Author(s):
YAO Pengqiang; QU Xuelian; CHENG Shiping
(School of Chemical and Environment Engineering,Pingdingshan University,Pingdingshan,Henan 467000)
关键词:
野生猕猴桃; 离体培养; 体细胞染色体加倍; 同源四倍体
Keywords:
wild kiwifruit; leaves in vitro; somatic chromosome doubling; autotetraploid
DOI:
10.11937/bfyy.20194173
文献标志码:
A
摘要:
以河南省平顶山市伏牛山区野生猕猴桃为试材，采用秋水仙碱诱导离体叶片体细胞染色体加倍的方法，研究了不同预培养时间以及不同秋水仙碱浓度对四倍体诱导效率的影响，以期获得野生猕猴桃同源四倍体诱导的最佳条件。结果表明：猕猴桃叶片不定芽诱导的最适培养基为MS+3.0 mg?L-1 6-BA+0.2 mg?L-1 NAA，叶片分化率可达88.00%；不定芽生根的最适培养基为1/2 MS+1.0 mg?L-1 IBA，生根率可达86.67%。四倍体诱导最佳处理组合为预培养3 d的离体叶片在含有50 mg?L-1的秋水仙碱培养基中浸泡72 h，四倍体植株诱导率最高为18.52%，共获得19个四倍体植株，继代培养3次后进行倍性检测，其倍性水平仍保持稳定。
Abstract:
Wild kiwifruit were used as materials which located in Funiu mountain,Pingdingshan city,Henan Province.The method of using colchicine to induce the somatic chromosome doubling in leaves in vitro was adopted.The effects of different preculture time and colchicine solution to the induction efficiency of autotetraploid were investigated.This research try to get the best induction condition of autotetraploid for kiwifruit.The results showed that the suitable induction culture medium of adventitious bud was MS+3.0 mg?L-1 6-BA+0.2 mg?L-1 NAA with 88.00% differentiation rate.The suitable culture medium of rooting was 1/2MS+1.0 mg?L-1 IBA with 86.67% rooting rate.The optimal treatment combination of tetraploid induction was 50 mg?L-1 colchicine solution with 72 hours culture after three days preculture leaves.The induction rate was about 18.52%.19 tetraploids were induced by this method.The ploidy level could remain stable after three subculture.

参考文献/References:

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备注/Memo

第一作者简介：姚鹏强（1986-），男，博士，讲师，现主要从事植物遗传育种等研究工作。E-mail:2779@pdsu.edu.cn.责任作者：程世平（1985-），男，博士，讲师，现主要从事植物遗传育种等研究工作。E-mail:shipingcheng@163.com.基金项目：平顶山学院高层次人才科研启动资助项目（BXY-BSQD-2018031，PXY-BSQD2016009）；河南省科技攻关计划资助项目（182102110132，172102110111，NBTZ201801）。收稿日期：2019-11-05

更新日期/Last Update: 2020-10-28