WANG Yushu,SHEN Jingyi,GENG Wei,et al.Callus Induction and Preventing Browning for Small Watermelon in Anther Culture[J].Northern Horticulture,2018,42(10):41-44.[doi:10.11937/bfyy.20180228]
小型西瓜花药愈伤组织诱导及防褐化研究
- Title:
- Callus Induction and Preventing Browning for Small Watermelon in Anther Culture
- 文献标志码:
- A
- 摘要:
- 以8份不同基因型的小型西瓜为试材，研究了基因型、植物生长调节剂对花药愈伤组织诱导的影响及暗培养和抗坏血酸对花药褐化的影响，以期为西瓜单倍体育种提供参考依据。结果表明：8份西瓜材料中7个能够形成愈伤组织，愈伤组织诱导率为3.9%~35.6%；不同基因型对激素的响应不同,‘P15’在D5号培养基中（MS＋KT 1.0 mg?L-1＋NAA 0.5 mg?L-1）愈伤组织诱导率最高,‘M30’和‘M34’在D6号培养基中（MS＋KT 1.0 mg?L-1＋NAA 1.0 mg?L-1）出愈率最高；黑暗培养48 h对降低西瓜花药褐化作用最好；在诱导培养基中添加1.0 g?L-1 抗坏血酸可降低花药褐化率。
- Abstract:
- The anthers of eight genotypes watermelon were used as test materials to investigate the effect of genotype and plant growth regulator on callus induction，and effect of dark culture and ascorbic acid on anther browning,in order to provide reference for the haploid breeding of watermelon.The results showed that 7 of 8 watermelon materials could form callus and the callus induction rate was from 3.9% to 35.6%.The response of different genotypes to hormones was different,and the callus induction of ‘P15’ was the highest in the D5 medium (MS+KT 1.0 mg?L-1+NAA 0.5 mg?L-1),and the rate of ‘M30’ and ‘M34’ was the highest in the D6 medium (MS+KT 1.0 mg?L-1+NAA 1.0 mg?L-1).The dark culture for 48 hours was the best to reduce the browning effect of watermelon anther.The addition of 1.0 g?L-1 ascorbic acid in the induction medium could reduce the rate of anther browning.
参考文献/References:
[1]孙胜,邢国明.中国小型西瓜反季节栽培研究进展[J].中国农学通报,2005,21(5):316-319. [2]赖黎丽,张嘉园,杨梅,等.新疆加工型辣椒花药培养技术研究[J].北方园艺,2017(19):6-10. [3]王炜,陈琛,欧巧明,等.小麦花药培养的研究和应用[J].核农学报,2016,30(12):2343-2354. [4]薛光荣,余文炎,费开伟,等.西瓜花药离体培养获得花粉植株[J].植物生理学通讯,1983(4):40-42． [5]苏维,常彩涛.辣椒单倍体培养研究进展[J].天津农业科学,2010,16(4):11-14． [6]柳美南,钟海明,陈绵桥,等.水稻花药培养及其应用[J].现代农业科技,2008(17):234． [7]朱迎春,刘君璞,邓云，等.不同因素对西瓜花药愈伤组织诱导的影响[J].河南农业科学,2015,44(12):104-111. [8]王仲慧,刘金兵,刁卫平,等.辣椒花药培养胚状体发生途径影响因子研究[J].核农学报,2015(8):1471-1478. [9]范建新,邓仁菊,王永清,等.火龙果花药培养诱导胚性愈伤组织及遗传稳定性[J].分子植物育种,2017,15(1):258-266. [10]张俊琦,罗晓芳.牡丹组织培养中褐化的发生原因与防止方法的研究[J].沈阳农业大学学报,2006,37(5):720-724. [11]张庆霞.陇东海棠初代离体培养中外植体褐化影响因素研究[J].中国南方果树,2016,45(5):117-120. [12]蒋益虹.百合褐变与多酚氧化酶和过氧化物酶活性关系的研究[J].浙江大学学报,2003,29(5):518-522.
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备注/Memo
第一作者简介：王玉书（1985-），女，博士，副教授，研究方向为园艺作物遗传与分子育种。E-mail：wangys1019@126.com.责任作者：高美玲（1978-），女，博士，教授，硕士生导师，研究方向为西甜瓜遗传育种及生物技术。Ｅ-mail:gaomeiling0539@163.com.基金项目：国家自然科学基金资助项目(31401908，31772334，31401891);黑龙江省自然科学基金资助项目(C2016056)；黑龙江省普通高等学校青年创新人才培养计划资助项目(UNPYSCT-2017155);黑龙江省省属高等学校基本科研业务费专项资助项目(135209313，135209106);齐齐哈尔大学大学生创新创业资助项目（210128）。收稿日期：2018-02-28