GOU Yan-xia,ZHANG Ming-fang.Study on Factors Affecting Anther Culture in virto of Citrullus lanatus[J].Northern Horticulture,2013,37(10):117-120.
西瓜花药离体培养影响因子研究
- Title:
- Study on Factors Affecting Anther Culture in virto of Citrullus lanatus
- 文章编号:
- 1001-0009(2013)10-0117-04
- Keywords:
- Citrullus lanatus; anther culture; callus induction
- 分类号:
- S 651
- 文献标志码:
- A
- 摘要:
- 以具有较多优良性状的F1代西瓜品种“春光”、“拿比特”和“喜都”的花药为试材，研究了影响西瓜花药愈伤组织诱导率的因素。结果表明：西瓜花药在MS+KT 1.5 mg/L+BA 2.0 mg/L+NAA 1.5 mg/L上诱导率最高;固体培养时蔗糖浓度以6%效果较好;4℃下低温预处理72 h可以提高西瓜花药愈伤组织的诱导率;30℃下高温预培养72 h后置于25℃低温下培养也可提西瓜花药愈伤组织的诱导率;黑暗条件下比较容易诱导出质地良好的西瓜花药愈伤组织。
- Abstract:
- Using three Citrullus lanatus cultivars of F1generation ‘Chunguang’，‘Nabite’，‘Xidu’ which including lots of merits as materials，the technology of anther culture were studied.The results showed that more callus were formed on MS+KT 1.5 mg/L+BA 2.0 mg/L+NAA 1.5 mg/L；the best concentration of sucrose for callus induction was 6%；the results indicated that the treating of 4℃，72 hours was the proper condition that make the frequencies of callus induction；the studying of culture conditions and culture means indicated that callus inducement was better when it was cultured at 30℃ for 72 h and then cultured at 25℃.The quality of watermelon callus induced in dark was much better than in light.
参考文献/References:
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备注/Memo
第一作者简介：缑艳霞(1978-)，女，硕士，讲师，研究方向为西甜瓜遗传育种改良。
收稿日期：2013-01-16