ZHU Xiaoqin,ZHANG Han,LIU Shilong,et al.Effects of Arbuscular Mycorrhizal Fungi on Growth of Strawberry Under p-hydroxybenzoic Acid Stress[J].Northern Horticulture,2017,41(14):44-50.[doi:10.11937/bfyy.20170284]
丛枝菌根真菌缓解对羟基苯甲酸抑制草莓生长的作用
- Title:
- Effects of Arbuscular Mycorrhizal Fungi on Growth of Strawberry Under p-hydroxybenzoic Acid Stress
- Keywords:
- strawberry(Fragaria ananassa Duch.); p-hydroxybenzoic acid; arbuscular mycorrhizal fungi; photosynthesis; root system; antioxidant enzymes activity
- 文献标志码:
- A
- 摘要:
- 以草莓为试材，采用接种丛枝菌根 (AM) 真菌和添加对羟基苯甲酸 (PHBA) 的方法，研究了根内球囊霉和摩西球囊霉对草莓自毒物质对羟基苯甲酸胁迫的缓解作用，即对草莓光合作用、根系结构和抗氧化物酶活性的影响。结果表明：0.5 mmol?L-1 PHBA浓度时，接种根内球囊霉显著提高了草莓净光合速率、气孔导度、胞间CO2浓度和蒸腾速率；摩西球囊霉显著提高了草莓的净光合速率、胞间CO2浓度和瞬时水分利用效率；根内球囊霉明显提高了草莓实际光化学效率 (ФPSII)、荧光淬灭系数(qP)、电子传递速率 (ETR)；摩西球囊霉提高了ФPSII和ETR。0.5 mmol?L-1 PHBA浓度时，根内球囊霉明显增加了草莓根长、根投影面积、根表面积、根体积、根尖数、根分叉数，摩西球囊霉显著增加了根长和根尖数。0.5 mmol?L-1 PHBA胁迫下，2株AM真菌均明显提高了草莓根部超氧化物歧化酶、过氧化物酶、过氧化氢酶活性。2株AM真菌通过增强草莓光合能力、根系发育和提高抗氧化物酶活性缓解了PHBA对草莓的毒害作用，根内球囊霉改善根系结构和提高抗氧化物酶活性的效果较摩西球囊霉明显，可能与菌根侵染率相关。
- Abstract:
- Fragaria ananassa Duch.was used as material,the effects of arbuscular mycorrhizal (AM) fungi Rhizophagus irregularis and Funneliformis mosseae on photosynthesis，root system，and antioxidant enzymes of strawberry under p-hydroxybenzoic acid (PHBA) stress were investigated.The results showed that，under 0.5 mmol?L-1 PHBA concentration，Rhizophagus irregularis enhanced the net photosynthetic rate (Pn)，stomatal conductance，intercellular CO2 concentration (Ci)，and transpiration rate；Funneliformis mosseae increase Pn，Ci，and water-use efficiency.Under 0.5 mmol?L-1 PHBA concentration，Rhizophagus irregularis enhanced effective quantum yield of PSII (ФPSII)，photochemical quenching coefficient(qP) and photosynthetic electron transport rate (ETR).Funneliformis mosseae increased ФPSII and ETR.Under 0.5 mmol?L-1 PHBA stress，Rhizophagus irregularis greatly improved the root system，including the root length，surface，volume，tips，forks.Under 0.5 mmol?L-1 PHBA stress，Rhizophagus irregularis and Funneliformis mosseae significantly increased the activities of superoxide dismutase，peroxidase，and catalase in strawberry root.AM fungi alleviated PHBA stress on strawberry by the higher Pn，ФPSII，ETR and greater root system，and the higher activities of antioxidant enzymes than non-mycorrhizal seedlings.Strawberry inoculated with Rhizophagus irregularis had the greater root system and higher activities of antioxidant enzymes than those of seedlings colonized with Funneliformis mosseae，it might be Rhizophagus irregularis had the higher colonization in strawberry.
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备注/Memo
第一作者简介：朱晓琴（1975-），女，博士，讲师，研究方向为植物微生物互作。E-mail:zhuxq320@126.com.基金项目：国家自然科学基金资助项目（31571997）；河南省科技攻关资助项目（142102110185）；河南省高等学校重点科研资助项目（15A210045）。