YUAN Liuxiang,LI Houhua,FU Linjiang,et al.Tissue Culture Techniques of Neillia thyrsiflora[J].Northern Horticulture,2015,39(24):87-89.[doi:10.11937/bfyy.201524025]
绣线梅组织培养技术
- Title:
- Tissue Culture Techniques of Neillia thyrsiflora
- Keywords:
- Neillia thyrsiflora; tissue culture; stem segment
- 文献标志码:
- A
- 摘要:
- 以绣线梅（Neillia thyrsiflora）的茎尖或带腋芽的茎段为外植体，采用组织培养技术，研究了绣线梅快速繁殖体系。结果表明：芽诱导最适培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L，诱导率为90%；MS+6-BA 2.0 mg/L+NAA 0.2 mg/L为最佳增殖培养基，增殖系数达7.5；1/2MS+IBA 0.2 mg/L为最佳生根培养基，生根率66.7%。
- Abstract:
- Taking stem tips and internodes with axillary buds of Neillia thyrsiflora as the explants，the rapid propagation system in vitro was studied by tissue culture techniques.The results showed that MS+6-BA 1.0 mg/L+NAA 0.2 mg/L was the suitable medium for explants induction of cluster buds,induction rate reached 90%；MS+6-BA 2.0 mg/L+NAA 0.2 mg/L was the optimizing medium for multi-propagation，and its average proliferation coefficient reached 7.5；the optimal rooting medium of shoots was 1/2MS+IBA 0.2 mg/L，the rooting rate was 66.7%.
参考文献/References:
[1]中国科学院西北植物研究所.秦岭植物志[M].北京：科学出版社,1981. [2]任学敏,李思锋,黎斌,等.秦岭山地主要野生木本观赏植物资源评价[J].西北林学院学报，2013，28(5):71-78. [3]蒙玉霞,张仲举,刘馨湦.中华绣线梅扦插繁育技术试验[J].陕西农业科学,2013(5):114-116. [4]OH S H.A systematic study of tribe neillieae (Rosaceae) [D].California:University of California，2002. [5]OH S H，POTTER D.Phylogenetic utility of the second intron of LEAFY in Neillia and Stephanandra (Rosaceae) and implications for the origin of Stephanandra[J].Molecular Phylogenetics and Evolution，2003，29(2)：203-215. [6]许红梅.植物组织培养中的污染及防止措施[J].北方园艺,2006(6):148-149. [7]周俊辉,周厚高,刘花全.植物组织培养中的内生细菌污染问题[J].广西植物,2003,23(1):41-47. [8]LEIFERT C，RITCHIE J Y，WAITES W M.Contaminants of plant-tissue and cell cultures [J].World Journal of Microbiology and Biotechnology,1991,7(4):452-468. [9]陈耀锋.植物组织与细胞培养[M].北京:中国农业出版社,2007.
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备注/Memo
第一作者简介：袁柳祥(1991-)，男，硕士研究生,研究方向为园林植物分子生物学。E-mail：961004274@qq.com.责任作者：李厚华（1973-），男，博士，副教授,现主要从事植物基因工程和类黄酮次生代谢等研究工作。E-mail：lihouhua73@163.com.基金项目：国家林业公益性行业科研专项资助项目（201204308）；西北农林科技大学基本科研业务费资助项目（QN2013079）。