LIU Min,FENG Dongdong,LU Hong,et al.Study on the Optimization of Liquid Cultural Medium for Tricholoma lobayense Heim[J].Northern Horticulture,2015,39(14):152-155.[doi:10.11937/bfyy.201514039]
金福菇液体培养基的优化研究
- Title:
- Study on the Optimization of Liquid Cultural Medium for Tricholoma lobayense Heim
- Keywords:
- Tricholoma lobayense Heim; liquid strain; culture medium; optimization
- 文献标志码:
- A
- 摘要:
- 以金福菇为试材,采用液体摇瓶培养法,以菌丝生物量为主要指标,通过单因素试验、正交实验对金福菇液体菌种培养基配方进行研究。结果表明:最适宜的培养基配方为马铃薯2.0%、葡萄糖3.0%、麸皮2.5%、酵母膏0.3%、KH2PO4 0.1%、MgSO4?7H2O 0.1%。
- Abstract:
- Taking Tricholoma lobayense Heim as test material,the liquid spawn medium formula of Tricholoma lobayense Hiem in submerged culture with shake flask were studied according to the index of mycelium fresh weight by single factor experiment and quadrature experiment.The results showed that the optimal medium of liquid spawn was as follows:potatoes 2.0%,glucose 3.0%,bran 2.5%,yeast extract 0.3%,KH2PO4 0.1%,MgSO4?7H2O 0.1%.
参考文献/References:
[1]傅俊生,蔡衍山,柯丽娜,等.金福菇的交配型研究[J].食用菌学报,2007,14(3):10-12. [2]陈成弟.金福菇优质高质栽培技术[J].热带农业,2001(4):3. [3]卫松海,周根红,杜适普,等.作物秸杆栽培金福菇技术[J].河南农业,2004(10):27. [4]许海涛.金福菇及其高产栽培技术[J].北京农业,2002(7):17-18. [5]杨庆尧.食用菇的深层发酵[J].中国食用菌,1985(5):6-8. [6]陈陶声.食用菌的栽培和发酵[M].北京:化学工业出版社,1988:127-155. [7]王谦,闫蕾蕾,王永利,等.金顶侧耳的深层液体培养及相关检测[J].菌物系统,2002,21(1):102-106. [8]甄世梅,杨树德,于兰兰,等.金福菇液体发酵培养基的优化[J].食用菌,2010(7):92-95.
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[J].Northern Horticulture,2016,40(14):152.[doi:10.11937/bfyy.201613039]
备注/Memo
第一作者简介:刘敏(1985-),女,硕士,实验师,现主要从事食药用真菌研究与开发等研究工作。E-mail:liumin870115@163.com.基金项目:河北省现代农业产业技术体系食用菌创新团队资助项目(冀农科发[2014]33号)。