LI Min,LUO Yiyuan,YUE Qibo.Study on Optimizing Extraction Techniques ofPotentilla chinensis Ser.Polysaccharides by Response Surface Methodology[J].Northern Horticulture,2015,39(14):147-151.[doi:10.11937/bfyy.201514038]
响应面法优化委陵菜多糖提取工艺研究
- Title:
- Study on Optimizing Extraction Techniques ofPotentilla chinensis Ser.Polysaccharides by Response Surface Methodology
- 文献标志码:
- A
- 摘要:
- 以委陵菜为试材，以多糖的提取率为响应值，分别选取液料比、超声功率、超声温度、提取时间4个因素进行Box-Behnken中心组合设计，通过响应面分析法优化委陵菜多糖提取工艺。结果表明：委陵菜总多糖的最佳提取工艺条件为液料比50∶1 mL/g，超声温度 63℃，超声功率500 W，提取时间30 min。其多糖的提取率为2.448 6%。响应面分析法用于提取工艺的优化，方法简单，具有可行性。
- Abstract:
- Taking Potentilla chinensis Ser.as test material，with the index for the content of polysaccharides in Potentilla chinensis Ser.,the effect of four factors such as solvent-solid ratio，ultrasonic power，ultrasonic temperature and extraction time were designed by Box-Behnken central composite.Extraction technic parameters of polysaccharides in Potentilla chinensis Ser.was optimized by response surface methodology.The results showed that the optimizing extraction conditions of polysaccharides in Potentilla chinensis Ser.were as follows：liquid-solid ratio was 50∶1 mL/g，ultrasonic power was 500 W，ultrasonic temperature was 63℃ and extraction time was 30 minutes.Under the conditions the extraction rate of polysaccharides was 2.448 6%.Optimizing extraction technics by response surface methodology was reasonable，simple，and had good feasibility.
参考文献/References:
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备注/Memo
第一作者简介：李敏（1984-），女，硕士，讲师，研究方向为天然药物化学。E-mail：limin1042004@163.com.基金项目：长白山动植物资源利用与保护吉林省高校重点实验室资助项目（吉农院合字2013第S012号）。