QIN Xiu-li,XING Li,YIN Rui.Study on the Key Cultivation Technology of North Cordyceps Host[J].Northern Horticulture,2013,37(17):157-160.
北冬虫夏草寄主体培养关键技术研究
- Title:
- Study on the Key Cultivation Technology of North Cordyceps Host
- 文章编号:
- 1001-0009(2013)17-0157-04
- Keywords:
- North Cordyceps; liquid strain; hyphae growth; pupa rigid rate; sporocarp growth
- 分类号:
- S 567
- 文献标志码:
- A
- 摘要:
- 以野生北冬虫夏草为试材,研究了北冬虫夏草寄主体培养关键技术。结果表明:液体菌种的最佳菌龄为7~9 d,用臭氧水进行蛹体表面消毒其效果最佳,接种时在蚕蛹翅的下缘进行接种易操作,出血少,僵化率高;通过正交实验确定发菌时适宜的条件为温度20~22℃,空气湿度60%,在无光条件下进行发菌,其僵化率最高;出草的适宜条件为温度20~22℃,空气湿度60%~70%,光照强度250~500 lx,其蛹虫草长势最好,得到的蛹虫草干重高。
- Abstract:
- Taking wild North Cordyceps as material,the key cultivation technology of North Cordyceps host was conducted.The results showed that the best bacteria age of liquid strains was 7~9 d,ozone solution was the best surface disinfection of pupa body,it was easy operation by inoculate on the lower edge of Silkworm pupa wing and bleeding less,with high rigid rate.Suitable conditions were determined by the orthogonal test,the results showed that the suitable temperature for the hyphae growth was 20~22℃,relative air humidity was 60%,the pupa rigid rate was the highest in no light condition.The suitable conditions for the sporocarp growth as follows,temperature for 20~22℃,relative air humidity for 60%~70%,light density for 250~500 lx,under this condition,the North Cordyceps growth was the best,the dry weight of North Cordyceps was the highest.
参考文献/References:
[1] 曾宏彬,宋斌,李泰辉,等.蛹虫草研究进展及其产业化前景[J].食用菌学报,2011,18(2):70-74.
[2] 李军,陈广生,方清茂,等.人工培养北冬虫夏草与冬虫夏草的比较研究[J].成都中医药大学学报,2010,33(3):82-84.
[3] Kim Y C.Cordyceps sinensis[J].American Journal of Chinese Medicine,1996,24(2):111-125.
[4] Ionnidis P,Courtis N,Havredaki M,et al.The polyadenylation inhibitor cordycepin cause a decline in c-MYC mRNA levels without effecting c-MYC protein level[J].Oncogene,1999,18(1):117-125.
[5] Zhou X X,Meyer C U,Schmidtke P,etal.Effect of cordycepin on interleukin-10 productions of human peripheral blood mononuclear cells[J].European Journal of Pharmacology,2002,453:309-317.
[6] 张显科,王玉柱,刘文霞.蛹虫草与冬虫夏草化学成分比较[J].辽宁大学学报,1996,23(4):20-23.
[7] 张平,朱述钧,钱大顺,等.北冬虫夏草功能成分及保健作用分析[J].江苏农业科学,2003(6):105-107.
[8] 金凌云,杜双田,马璐,等.蛹虫基质的模型优化研草栽培[J].西北农林科技大学,2009,37(11):155-159.
[9] 王兰珍,林桂荣.不同培养条件对北冬虫夏草原基形成的影响[J].福建农业科技,2009(4):44-47.
[10] 林群英,宋斌,钟月金,等.北冬虫夏草人工栽培条件优化研究[J].中国食用菌,2006,25(6):17-18.
[11] 穆忠平.蛹虫草高产培育技术[J].食用菌,2004(5):36-37.
[12] 秦秀丽,杨国会,李凤林.北冬虫夏草液体深层发酵的研究[J].北方园艺,2010,23(6):167-170.
[13] 马文石,李凤芹,金英.柞蚕北冬虫夏草栽培技术[J].食用菌,2008(4):45-46.
相似文献/References:
[1]郭欣欣,樊淑华,王永立.金针菇液体培养基的优化研究[J].北方园艺,2014,38(02):146.
GUO Xin-xin,FAN Shu-hua,WANG Yong-li.Study on the Optimization of Liquid Cultural Medium of Flammulina velutipes [J].Northern Horticulture,2014,38(17):146.
[2]董玉兰,李书生,张丽萍,等.白灵菇高活力液体菌种摇瓶发酵培养条件的优化[J].北方园艺,2014,38(06):143.
DONG Yu-lan,LI Shu-sheng,ZHANG Li-ping,et al.The Optimization of the High Energy Liquid Culture Conditions of Pleurotus nebrodensis[J].Northern Horticulture,2014,38(17):143.
[3]杨丽维,王玉,班立桐,等.杏鲍菇液体菌种培养基的筛选和优化[J].北方园艺,2014,38(06):150.
YANG Li-wei,WANG Yu,BAN Li-tong,et al.Study on the Screening and Optimizing of Liquid Spawn Medium of Pleurotus eryngii[J].Northern Horticulture,2014,38(17):150.
[4]秦秀丽,邢 力,尹 锐.北冬虫夏草人工培养固体培养基优化研究[J].北方园艺,2013,37(06):149.
QIN Xiu-li,XING Li,YIN Rui.Study on Artificial Cultivation Optimization of Solid Culture Medium of Cordyceps militaris [J].Northern Horticulture,2013,37(17):149.
[5]韦 珂,李丽瑛,覃培升,等.榆黄蘑液体菌种培养研究[J].北方园艺,2013,37(20):131.
WEI Ke,LI Li-ying,QIN Pei-sheng,et al.Study on Culture of Liquid Strain of Pleurotus citrinopileatus[J].Northern Horticulture,2013,37(17):131.
[6]郭明月,王琼波,魏永义.低糖虫草复合保健饮料的研制[J].北方园艺,2012,36(06):164.
GUO Ming-yue,WANG Qiong-bo,WEI Yong-yi.Study on Low-sugar Compound Healthy Beverage with Cordyceps militaris[J].Northern Horticulture,2012,36(17):164.
[7]秦秀丽,邢力,尹锐,等.北冬虫夏草菌种的选育及生物学特性研究[J].北方园艺,2012,36(09):175.
QIN Xiu-li,XING Li,YIN Rui,et al.Study on the Breeding and Biological Characteristics of Cordyceps Strains[J].Northern Horticulture,2012,36(17):175.
[8]陈帅君,黄亮,王玉,等.棕色蘑菇菌丝液体培养基的优化[J].北方园艺,2015,39(08):150.[doi:10.11937/bfyy.201508039]
CHEN Shuai-jun,HUANG Liang,WANG Yu,et al.The Optimization of Liquid Medium for Mycelium Culture of Agaricus brunnescens[J].Northern Horticulture,2015,39(17):150.[doi:10.11937/bfyy.201508039]
[9]王谦,郭苗,刘敏,等.一种以黄浆水为原料制备白灵菇液体菌种的方法[J].北方园艺,2015,39(09):115.[doi:10.11937/bfyy.201509032]
WANG Qian,GUO Miao,LIU Min,et al.A Method Employ Waste Material of Nejayote as Raw Material to Prepare Pleurotus nebrodensia Liquid Spawn[J].Northern Horticulture,2015,39(17):115.[doi:10.11937/bfyy.201509032]
[10]刘敏,冯东东,卢红,等.金福菇液体培养基的优化研究[J].北方园艺,2015,39(14):152.[doi:10.11937/bfyy.201514039]
LIU Min,FENG Dongdong,LU Hong,et al.Study on the Optimization of Liquid Cultural Medium for Tricholoma lobayense Heim[J].Northern Horticulture,2015,39(17):152.[doi:10.11937/bfyy.201514039]
备注/Memo
第一作者简介:秦秀丽(1966-),女,本科,副教授,现主要从事微生物及食药用菌的教学与科研工作。E-mail:qinxiuli88@126.com.
基金项目:吉林省教育厅“十一五”科学技术研究资助项目(吉教科合字[2011]第274号);
吉林省科技厅发展计划资助项目(201105071)。
收稿日期:2013-04-22