WEI Ke,LI Li-ying,QIN Pei-sheng,et al.Study on Culture of Liquid Strain of Pleurotus citrinopileatus[J].Northern Horticulture,2013,37(20):131-134.
榆黄蘑液体菌种培养研究
- Title:
- Study on Culture of Liquid Strain of Pleurotus citrinopileatus
- 文章编号:
- 1001-0009(2013)20-0131-04
- Keywords:
- Pleurotus citrinopileatus; liquid strain; carbon source
- 分类号:
- S 646.1+4
- 文献标志码:
- A
- 摘要:
- 以榆黄蘑原种、栽培种为试材，采用摇瓶培养榆黄蘑液体菌种，通过单因子试验筛选较优的速效碳源和迟效碳源，较优的复合碳源比、碳氮比、培养时间，并通过回管法进行菌种落发试验。结果表明：较优的速效碳源和迟效碳源分别为麦芽糊精和玉米淀粉，较优的速效碳源和迟效碳源比例为1∶2，较优的碳氮比为12∶1，最适培养时间为5 d，采用此条件制备的液体菌种栽培榆黄蘑，菌丝满袋时间较固体菌种减少1~2 d，生物学效率与固体接种相近。
- Abstract:
- Taking original species and cultivars of Pleurotus citrinopileatus as test materials，using shake flasks citrinopileatus liquid spawn,the more fast-acting and slow-acting carbon source ratio,complex carbon source ratio,carbon-nitrogen ratio,culture time were screened by single factor experiment,and strain germination experiment was done by return pipe method.The results showed that the best more fast-acting and slow-acting carbon carbon were maltodextrin and corn starch.Acting on better carbon and delayed-carbon ratio was 1∶2，and more of the carbon-nitrogen ratio of 12∶1；optimal culture time was 5 d；using this condition citrinopileatus liquid spawn cultivation，bacteria solid silk bag full of bacteria decreased over 1~2 d，biological efficiency and solid vaccination similar.
参考文献/References:
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备注/Memo
第一作者简介：韦珂（1972-），女，博士，副教授，现主要从事应用微生物研究工作。E-mail：weikeflower@gxu.edu.cn.