XIA Ya-nan,JIANG Jian-xiong,YI Zi-li,et al.Effect of Three Antioxidants on Browning of Explant and Callus Induction Rate of Miscanthus lutarioriparius[J].Northern Horticulture,2014,38(17):93-96.
三种抗褐化剂对南荻外植体褐变及愈伤诱导率的影响
- Title:
- Effect of Three Antioxidants on Browning of Explant and Callus Induction Rate of Miscanthus lutarioriparius
- 文章编号:
- 1001-0009(2014)17-0093-04
- Keywords:
- Miscanthus lutarioriparius; tissue culture; browning; polyvinylpyrrolidone; vitamin C; citric acid
- 分类号:
- Q813.1+2
- 文献标志码:
- A
- 摘要:
- 以能源植物南荻的幼穗为外植体，MS为基本培养基，通过在诱导培养基中分别添加一定浓度梯度的聚乙烯吡咯烷酮（polyvinylpyrrolidone,PVP）、维生素C(vitamin C)及柠檬酸(citric acid,CA），对比研究3种抗褐化剂对南荻外植体褐变及愈伤诱导率的影响。结果表明：在诱导培养基中，添加PVP、维生素C和CA均能减轻外植体的褐化程度；相对于对照（CK）35.83%的诱导率，在试验浓度范围内，添加PVP、维生素C和CA后愈伤组织诱导率可分别提高至55.42%、50.42%和124.00%,CA处理组与对照组之间愈伤诱导率差异达极显著水平（P<0.01）。因此，CA为南荻幼穗愈伤组织诱导的最佳抗褐化剂，且浓度为0.2 g/L时诱导率显著提高2.46倍。
- Abstract:
- Taking the immature inflorescences of M.Lutarioriparius as explants and MS medium with polyvinylpyrrolidone (PVP),vitamin C or citric acid (CA) were used as medium in this research,the effect of 3 antioxidants to inhibit browning of explants and on callus induction rate of Miscanthus lutarioriparius were studied.The results showed that treating with PVP,vitamin C and CA could improve callus induction rate and reduce the browning of explants in a certain degree.Compared with the control whose callus induction rate were 35.83%,adding PVP,vitamin C or CA in the medium increased the induction rate to 55.42%,50.42% and 124.00% respectivly；and adding CA made highly significant difference (P<0.01).CA was the best anti-browning agent of immature inflorescences culture of M.lutarioriparius,and 0.2 g/L CA could significantly increase the callus induction rate by 2.46 times.
参考文献/References:
［1］Chen S L,Renvoize S A.Miscanthus Andersson［M］//Wu Z Y,Raven P H.Flora of China.Beijing：Science Press,2006:581-583.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(17):106.
[4]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(17):80.
[5]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(17):84.
[6]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(17):101.
[7]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(17):89.
[8]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(17):95.
[9]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(17):106.
[10]陈丽文,杨利平,荣薏,等.金心也门铁工厂化育苗技术研究[J].北方园艺,2013,37(13):138.
CHEN Liwen,YANG Li-ping,RONG Yi,et al.Study on the Techniques of Industrialized Seedling Culture of Draceana arborea[J].Northern Horticulture,2013,37(17):138.
备注/Memo
第一作者简介：夏亚男（1989-），女，湖南益阳人，硕士研究生，研究方向为能源植物细胞遗传学。E-mail：xiayanan1213@163.com.