LIU Xiao-fei,LIAO Fei-xiong,LIU Xiao-rong,et al.Genetic Identification of Celosia Mutant and Select Lines Using Sequence-related Amplified Polymorphism，SRAP[J].Northern Horticulture,2013,37(07):128-130.
鸡冠花诱变选育系和栽培选育系的SRAP分子鉴定
- Title:
- Genetic Identification of Celosia Mutant and Select Lines Using Sequence-related Amplified Polymorphism，SRAP
- 文章编号:
- 1001-0009(2013)07-0128-03
- Keywords:
- Celosia cristata; SRAP; fingerprint
- 分类号:
- S 681.3
- 文献标志码:
- A
- 摘要:
- 以冠状鸡冠花“早水鸡冠”及其诱变选育系、羽状鸡冠印度系列中的2个品种为试材，应用SRAP分子标记技术分析其遗传多样性。结果表明：从64对引物组合中筛选出双引物组合me6/g38，具有遗传多态性，可在分子水平上区分这6种供试材料。NTSYS-pc聚类分析（UPGMA）构建了SRAP分子图谱，“早水鸡冠”及源自其的卫星搭载处理后经多代选择的3个诱变选育系可聚为一类，2个印度系品种聚为一类，表明SRAP可用于鸡冠花品种资源鉴定与遗传多样性分析。
- Abstract:
- Taking Celosia cristata ‘Zaoshuijiguan’ and its three selected lines from the mutants，two cultivars from C.plumos ‘Indian’ as materials,the genetic diversity using Sequence-related Amplified Polymorphism (SRAP) were studied.The results showed that one primer pairs me6/g38 from 64 primer pairs amplified stable polymorphism among the 6 varieties with 7 amplifications.All the 6 lines tested could be identified each other by the fingerprint map constituted using the amplifications from me6/g38.The results of Cluster analysis (UPGMA) based on this fingerprint map showed ‘Zaoshuijiguan’ and three mutation lines，which were carried by satellite and cultured for generation，could clustered into a group at cofficient 0.52，while two Indian lines were clustered into a groups at cofficient 0.84.It indicated that SRAP would be a potential molecular marker tool in germplasm identification and genetic diversity analysis of celosia.
参考文献/References:
[1]陈俊愉,程绪珂.中国花经[M].上海：上海文化出版社,1990:265. [2]Hase K，Basnet P，Kadota S，et al.Immuno stimulating activity of Celosian，an antihepatotaoxic polysaccharide isolated from Celosia argentea[J].Planta Med,1997,63:216-219. [3]Hayakawa Y，Fujii H，Hase K，et al.Antimetastatic and immunomodulating properties of the water extract from Celosia argentea seeds[J].BiolPharm Bull,1998,21:1154-1159. [4]Tadao A.Amino acids[M].Japan：The Japan essential amino acids association.Incorporated,1987：13-18. [5]姚悦梅,毛忠良,潘永飞,等.播种期对不同品种鸡冠花观赏性及结实性的影响[J].江西农业学报,2009,21(10):54-56. [6]姜一凡,徐维杰,廖飞雄,等.花卉空间诱变效应及育种研究进展[J].中国农学通报,2007,23(8):339-342. [7]夏瑾华.新型鸡冠花的品种选育及其遗传学研究[D].武汉:湖北大学,2004. [8]张继业，钟仙龙,李锡珍,等.60Co-r射线辐射鸡冠花种子对其发芽率及幼苗生长的影响[J].安徽农业科学,2005,33(4):620-720. [9]张孟锦,廖飞雄,王碧青,等.卫星搭载处理对鸡冠花SPl代种子萌发及生长发育的影响[J].广东农业科学,2006(4):37-39. [10]Li G，Quiros C F.Sequence-related amplified polymorpgism (SRAP) a new marker system based on a simple PCR reaction：its application to mapping and gene tagging in Brassica[J].Theor Appl Genet,2001,103:455-461. [11]王燕,龚义勤,赵统敏,等.番茄SRAP-PCR体系优化与品种分子鉴定[J].南京农业大学学报,2007,30(1):23-29. [12]任羽,王得元,张银东.辣椒SRAP-PCR反应体系的建立与优化[J].分子植物育种,2004,2(5):689-693. [13]吴洁,谭文芳,何俊蓉.甘薯SRAP连锁图构建淀粉含量QTL检测[J].分子植物育种,2005,3(6):841-845. [14]李严,张庆春.西瓜杂交种遗传多样性的SRAP标记分析[J].园艺学报,2005,32(4):643-647. [15]Feng N，Xue Q，Guo Q H，et al.Genetic diversity and population structure of Celosia argentea and related speciesrevealed by SRAP[J].Biochem Genet,2009,47:521-532. [16]姜一凡.卫星搭载鸡冠花后代的生物学效应及株系筛选[D].南昌:江西农业大学,2008. [17]Liu L，Guo W，Zhu X，et al.Inheritance and fine mapping of fertility restoration for cytoplasmic male sterility in Gossypium hirsutum?L[J].Theor Appl Genet,2003,106:461-469. [18]Sneath P H A，Sokal R R.Numerical taxonomy[C].Freeman,San Francisco,CA,1973.
相似文献/References:
[1]王晶,张晓明,闫国华,等.草原樱桃和对樱杂交后代的分子鉴定[J].北方园艺,2012,36(01):116.
WANG Jing,ZHANG Xiao-ming,YAN Guo-hua,et al.Molecular Identification of Hybrids between Prunus fruticosa and Prunus pseudocerasus by SRAP Method[J].Northern Horticulture,2012,36(07):116.
[2]郝凤,于铁峰,雷银川.番茄SRAP-PCR反应体系建立与优化[J].北方园艺,2012,36(03):107.
HAO Feng,YU Tie-feng,LEI Yin-chuan.Optimization for SRAP-PCR System of Tomato Based on Orthogonal Design[J].Northern Horticulture,2012,36(07):107.
[3]王骞春,黄夏,李光森.小干松SRAP-PCR反应体系的建立[J].北方园艺,2013,37(02):83.
WANG Qian-chun,HUANG Xia,LI Guang-sen.Establishment of the SRAP-PCR Reaction System of Pinus contorta[J].Northern Horticulture,2013,37(07):83.
[4]梁艳,杨晓杰,刘敏,等.鸡冠花的离体快繁及试管开花研究[J].北方园艺,2012,36(08):127.
LIANG Yan,YANG Xiao-jie,LIU Min,et al.Study on Rapid Propagation and Flowering of Cockscomb in vitro[J].Northern Horticulture,2012,36(07):127.
[5]裴毅,聂江力,刘会佳,等.NaCl和NaHCO3胁迫对鸡冠花种子萌发的影响[J].北方园艺,2014,38(22):65.
PEI Yi,NIE Jiang-li,LIU Hui-jia,et al.Effect of NaCl and NaHCO3 Stress on Seed Germination of Celosia cristata L.[J].Northern Horticulture,2014,38(07):65.
[6]刘 巍,陈 罡,颜廷武,等.美国红枫SRAP反应体系优化[J].北方园艺,2015,39(02):79.[doi:10.11937/bfyy.201502023]
LIU Wei,CHEN Gang,YAN Ting-wu,et al.Optimization of SRAP Reaction System in Acer rubrum[J].Northern Horticulture,2015,39(07):79.[doi:10.11937/bfyy.201502023]
[7]毛 伟,李培英,孙宗玖.偃麦草SRAP-PCR反应体系优化[J].北方园艺,2015,39(02):91.[doi:10.11937/bfyy.201502026]
MAO Wei,LI Pei-ying,SUN Zong-jiu.Optimization of SRAP-PCR System on Elytrigia repens[J].Northern Horticulture,2015,39(07):91.[doi:10.11937/bfyy.201502026]
[8]梁婷婷,马 燕,臧德奎.梨属野生资源遗传多样性的SRAP分析[J].北方园艺,2015,39(13):1.[doi:10.11937/bfyy.201513001]
LIANG Ting-ting,MA Yan,ZANG De-kui.Genetic Diversity Analysis of Wild Germplasm Resource of Pyrus Based on SRAP Markers[J].Northern Horticulture,2015,39(07):1.[doi:10.11937/bfyy.201513001]
[9]何 志 美,郭 军 战,包 振 华,等.离子注入诱变鸡冠花M1代形态变异的研究[J].北方园艺,2010,34(10):0.[doi:10.11937/bfyy.2010010056]
HEZhi -mei,GUO Jun -zhan,BAO Zhen -hua,et al.Morphological Variation in M1 Celosia cristata L Induced by Ion Implantation[J].Northern Horticulture,2010,34(07):0.[doi:10.11937/bfyy.2010010056]
[10]赵英,黄国弟,付海天,等.十二份杧果种质亲缘关系的SRAP分析[J].北方园艺,2015,39(21):99.[doi:10.11937/bfyy.201521026]
ZHAO Ying,HUANG Guodi,FU Haitian,et al.SRAP Analysis of 12 Mango Germplasm Genetic Relationships[J].Northern Horticulture,2015,39(07):99.[doi:10.11937/bfyy.201521026]
备注/Memo
第一作者简介：刘小飞（1984-），男，硕士，研究实习员，现主要从事花卉分子标记辅助育种等研究工作。E-mail:lxf6996@yahoo.com.cn.