LI Hong,LI Bo,WANG Ye,et al.Alfalfa Leaf Callus on Space Flight Induction and Response on Saline-alkali Stress[J].Northern Horticulture,2013,37(05):100-102.
卫星搭载苜蓿叶片愈伤组织对盐碱胁迫的响应
- Title:
- Alfalfa Leaf Callus on Space Flight Induction and Response on Saline-alkali Stress
- 文章编号:
- 1001-0009(2013)05-0100-03
- Keywords:
- alfalfa; callus; space flight; saline-alkali stress; physiological and biochemical indexes
- 分类号:
- S 551+.7
- 文献标志码:
- A
- 摘要:
- 以卫星搭载的苜蓿叶片为外植体，诱导产生愈伤组织，研究盐碱胁迫下苜蓿愈伤组织对盐和碱的半致死浓度和致死浓度，及其生理指标的变化。结果表明：苜蓿愈伤组织对NaCl的半致死浓度为1.2％，致死浓度为1.8％。对NaHCO3和Na2CO3的半致死浓度为0.4 mmol/L，致死浓度为1.0 mmol/L。卫星搭载提高了苜蓿愈伤组织POD活性、可溶性糖含量和脯氨酸含量，降低了丙二醛含量和相对电导率，差异显著。卫星搭载可以提高苜蓿愈伤组织对盐碱的抵抗能力，且对碱抵抗能力强于对盐的抵抗能力。
- Abstract:
- Taking alfalfa leaves on space flight as materials，the callus was induced.The half lethal concentration and lethal concentration of alfalfa callus under different concentrations of saline and alkali stress were studied，and 5 physiological and biochemical indexes under different saline-alkali stress were studied.The results showed that the half lethal and lethal concentration of alfalfa callus under NaCl stress were 1.2% and 1.8 mmol/L.The half lethal and lethal concentration of alfalfa callus under NaHCO3and Na2CO3stress were 0.4% and 1.0 mmol/L.The Alfalfa callus on space flight improved POD activity，soluble sugar and proline content，decreased the MDA content and relative conductivity，and the difference was significant.Space flight could improve saline alkali resistance of alfalfa callus，and alkali resistance was higher than that of salt resistance.
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备注/Memo
第一作者简介：李红（1960-），女，山东莱芜人，研究员，现主要从事牧草与饲料作物育种与栽培研究工作。E-mail:hljlihong@163.com.
基金项目：国家科技支撑计划资助项目（2011BAD17B01-04）；国家公益性行业科研专项资助项目（201303066）；黑龙江省教育厅科学技术研究资助项目（12521610）。
收稿日期：2012-11-06