[1]刘倩,康如龙,苏小建.高效液相色谱-蒸发光散射检测法测定果汁中单糖的含量[J].北方园艺,2012,36(21):36-38.
　LIU Qian,KANG Ru-long,SU Xiao-jian.Research on Free Sugars of Juice By HPLC-ELSD[J].Northern Horticulture,2012,36(21):36-38.
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高效液相色谱-蒸发光散射检测法测定果汁中单糖的含量

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年21期 页码: 36-38 栏目: 出版日期: 2012-11-15
Title:
Research on Free Sugars of Juice By HPLC-ELSD
文章编号:
1001-0009（2012）21-0036-03
作者:
刘倩1; 康如龙1; 苏小建2
1.广西师范大学 化学化工学院，广西 桂林 541004；
2.广西师范大学 环境与资源学院，广西 桂林 541004
Author(s):
LIU Qian1; KANG Ru-long1; SU Xiao-jian2
1.School of Chemistry and Chemical Engineering，Guangxi Normal University，Guilin，Guangxi 541004；
2.College of Environment and Resources，Guangxi Normal University，Guilin，Guangxi 541004
关键词:
西瓜; 游离糖; 高效液相色谱
Keywords:
watermelon; free sugars; HPLC
分类号:
TS 255.44
文献标志码:
A
摘要:
采用高效液相色谱（HPLC）对西瓜果汁中游离糖进行测定。结果表明：测定糖的最佳条件是色谱柱CLC-NH2（M），流速1.0 mL/min，柱温为室温，流动相为乙腈-超纯水（80∶20）;该方法的回收率在97.88%~99.35%，RSD为0.79%~3.62%。该方法简单、快速，适用于检测果汁中游离糖的含量。
Abstract:
Using HPLC method to determine the free sugars in watermelon juice.The optimum condition was as follows：Chromatographic column CLC-NH2(M)，flow rate 1.0 mL/min，column under the condition of room temperature and the mobile phase consisted of an aqueous solution of acetonitrile-ultra pure water(80∶20).The results showed that this method expressing high average recoveries ranged from 97.88% to 99.35% and good precision ranged from 0.79% to 3.62%.Free sugars in juice can be determined rapidly and effectively in this way.

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备注/Memo

第一作者简介：刘倩（1988-），女，在读硕士，研究方向为天然产物研究与开发。E-mail:liuqian1118@126.com.

责任作者：苏小建（1957-），男，本科，教授，硕士生导师，研究方向为天然产物研究与开发。E-mail:xiaojiangsu@163.com.
基金项目：广西专项科研基金资助项目。

更新日期/Last Update: 2014-09-01