CHEN Bo,CHEN Zhe-hao,WANG Li-lin,et al.Effects of Different Light Quality on the Formation and Regenerative Potentialities of Calli of Camptotheca acuminata[J].Northern Horticulture,2012,36(17):112-115.
不同光质对喜树愈伤组织形成及分化潜质影响
- Title:
- Effects of Different Light Quality on the Formation and Regenerative Potentialities of Calli of Camptotheca acuminata
- 文章编号:
- 1001-0009(2012)17-0112-04
- 分类号:
- S 792.119
- 文献标志码:
- A
- 摘要:
- 以喜树无菌苗的茎、叶为外植体，研究了蓝、白、红3种光质下，MS、1/2MS、SH、WPM 4种培养基对喜树愈伤组织形成及分化潜质影响。结果表明：蓝光下SH+1.0 mg/L NAA+0.075 mg/L TDZ培养基上愈伤组织诱导效果最好；愈伤组织继代后在蓝光下第7天时，SOD酶活最高，POD酶活最低，含水量最低，分化的潜力最大。
- Abstract:
- Taking the stems and leaves of Camptotheca acuminata as the explants,the formation and regenerative potentialities of calli from Camptotheca acuminata under different light quality(white/blue/red) and four media(SH,MS,WPM and 1/2MS) with 0.075 mg/L TDZ and 1.0 mg/L NAA were studied.The results showed that the calli cultured under blue light on SH medium had the best multiplication conditions.At the 7th day of subculture under blue light，the calli had the highest SOD activity and the lowest POD activity as well as water content，which might suggest a highly potential of regeneration of them.These results provided fundamental basis for the further research of genetic transformation，cell culture，plant regeneration and secondary metabolites acquisition in Camptotheca acuminata.
参考文献/References:
[1]Wall M E，Wani M C，Cookc E，et al.Plant Antitumor agent.I.the isolation and structure of camptothecin，a novel alkaloidal leukemia and tumor inhibitor from Camptotheca acuminate[J].J Am Chem Soc，1966,88(16):3888-3890. [2]姬惜珠,王红,张爱军.木本植物离体快繁中常见问题及解决方法[J].河北果树,2005(2):13-14. [3]陈颖,曹福亮,李淑娴,等.喜树茎段不定芽的诱导及再生系统的建立[J].经济林研究,2004,22(1):8-11. [4]吕立堂,朱冬雪,赵德刚.喜树茎尖组织培养与植株再生[J].中草药,2004,35(6):682-684. [5]郭勇.喜树愈伤组织和叶片中喜树碱含量变化研究[D].雅安:四川农业大学,2005. [6]Wang H M，Zu Y G，Wang W J，et al.Estabishment of Camptotheca acuminate regeneration from leaf explants[J].Biol Plant，2006,50(4):725-728. [7]Sang H L，Rajesh K T，Kee Y P.Photon flux density and light quality induce changes in growth，tomatal development，photosynthesis and transpiration of Withania somniferal(L.) Dunal plantlets[J].Plant Cell，Tissorgcult,2007,90(3):141-151. [8]刘媛,李胜,马绍英,等.肉桂酸和不同光质对葡萄愈伤组织增殖及白藜芦醇累计的效应[J].甘肃农业大学学报，2010,45(1):41-46. [9]刘浩，李胜，马绍英，等.LED不同光质对萝卜愈伤组织诱导、增殖及萝卜硫素含量的影响[J].植物生理学通讯，2010,46(4):347-350. [10]孙群,胡景江.植物生理学研究技术[M].杨凌:西北农林科技大学出版社,2006. [11]马林.喜树愈伤组织的诱导与培养[J].中国中药杂志,2007,32(13):1273-1276. [12]汪本勤.植物SOD的研究进展[J].河北农业科学,2008,12(3):6-9. [13]崔凯荣,任红旭,邢更妹,等.枸杞组织培养中抗氧化酶活性与体细胞胚发生相关性的研究[J].兰州大学学报(自然科学版),1998,34(3):93-99. [14]Burdon R H.Superoxide and hydrogen peroxide in relation to mammalian cell proliferation[J].Free Rad Biol and Med,1993(18):775-794. [15]田敏,韩凝,边红武,等.草莓愈伤组织再生能力与活性氧代谢水平相关性研究[J].园艺学报,2004,31(3):372-374. [16]汪晓谦,张延龙,牛立新,等.郁金香花芽分化过程中鳞茎碳水化合物和蛋白质含量的变化[J].植物生理学报,2011,47(4):379-384. [17]崔堂兵,郭勇,林炜铁.提高植物细胞培养法生产次级代谢物产量的方法[J].植物生理通讯,2001,37(5):479-482. [18]王爱民,肖炜,杜文雪,等.光质对缕丝花试管苗生长发育的影响[J].徐州师范大学学报(自然科学版),2001,19(4):56-58. [19]王亚丽,王晓东,赵兵,等.光质对玛咖愈伤组织生长、分化的影响[J].过程工程学报，2007,7(4):784-785. [20]林小苹,赖钟雄,黄浅.光质对植物离体培养的影响[J].亚热带农业研究,2008,4(1):73-80. [21]谢以萍,杨再强,苏天星,等.不同光质对瓜叶菊生长发育的影响[J].北方园艺,2010(3):53-56.
相似文献/References:
[1]陈祥伟,刘世琦,刘 庆,等.不同LED光源对小白菜生长及光合特性的影响[J].北方园艺,2013,37(22):1.
CHEN Xiang-wei,LIU Shi-qi,LIU Qing,et al.Effects of Different Light Qualities on Growth and Photosynthetic Characteristics of Pakchoi[J].Northern Horticulture,2013,37(17):1.
[2]李松丽,黄欢欢,蔡小东.两种凝固剂对冰糖橙愈伤组织增殖及植株再生的影响[J].北方园艺,2013,37(22):108.
LI Song-li,HUANG Huan-huan,CAI Xiao-dong.Effects of Two Types of Gelling Agents on the Callus Proliferation and Plant Regeneration of Citrus sinensis L.Osbeck cv.Bingtang[J].Northern Horticulture,2013,37(17):108.
[3]闫海霞,黄昌艳,何荆洲,等.观赏凤梨组织培养的影响因素分析[J].北方园艺,2013,37(22):196.
YAN Hai-xia,HUANG Chang-yan,HE Jing-zhou,et al.Analysis of the Influence Factors in Tissue Culture of Ornamental Bromeliads[J].Northern Horticulture,2013,37(17):196.
[4]王 婷,智永祺,周 坚.长筒石蒜叶片薄层培养诱导愈伤组织发生的研究[J].北方园艺,2014,38(01):91.
WANG Ting,ZHI Yong-qi,ZHOU Jian.Study on Callus Induction of the Leaf Blades of Lycoris longitubaUsing Thin Cell Layer Culture[J].Northern Horticulture,2014,38(17):91.
[5]焦战战,张东向,赵静,等.黄芩愈伤组织中黄芩苷与过氧化物酶活性相关性分析[J].北方园艺,2013,37(13):108.
JIAO Zhan-zhan,ZHANG Dong-xiang,ZHAO Jing,et al.Analysis of the Correlation Between Baicalin and Peroxidase Activity in the Callus of Scutellaria baicalensi Georgi[J].Northern Horticulture,2013,37(17):108.
[6]罗君琴,徐建国,王平.不同培养条件对柑桔胚性愈伤组织离体诱导的影响[J].北方园艺,2013,37(13):125.
LUO Jun-qin,XU Jian-guo,WANG Ping.Effect of Different Culture Condition on Embryogenic Callus in Vitro of Citrus[J].Northern Horticulture,2013,37(17):125.
[7]马少梅.北海道黄杨再生体系建立研究进展[J].北方园艺,2013,37(13):208.
MA Shao-mei.Research Advances on the Establishment of Regeneration System of Euonymus japonicus[J].Northern Horticulture,2013,37(17):208.
[8]于 娜,董宽虎.白羊草成熟种子组织培养再生体系研究[J].北方园艺,2014,38(02):104.
YU Na,DONG Kuan-hu.Research on Tissue Culture of Regeneration System of the Mature Seeds of Bothriochloa ischaemum [J].Northern Horticulture,2014,38(17):104.
[9]张丽霞,王毅彰.植物生长调节剂对花籽山药愈伤组织诱导形成的影响[J].北方园艺,2013,37(14):115.
ZHANG Li-xiaWANG Yi-zhang.Effect of Plant Growth Substances on Callus Induction of Seed Yam Forming[J].Northern Horticulture,2013,37(17):115.
[10]李金英,赵春莉,张馥茜,等.黑穗醋栗愈伤组织诱导研究[J].北方园艺,2013,37(17):87.
LI Jin-ying,ZHAO Chun-li,ZHANG Fu-qian,et al.Study on the Callus Induction of Blackcurrant[J].Northern Horticulture,2013,37(17):87.
[11]郭晓博,张晓丽,李俊华,等.山药愈伤组织研究进展[J].北方园艺,2014,38(24):183.
GUO Xiao-bo,ZHANG Xiao-li,LI Jun-hua,et al.Research Advances on Callus Production in Dioscorea opposite[J].Northern Horticulture,2014,38(17):183.
备注/Memo
第一作者简介：陈博（1986-），男，在读硕士，现主要从事植物遗传发育等研究工作。E-mail：chenbo19851128@163.com.