ZHANG Yong,FANG Yong-lin,DENG Li-jia,et al.Induction and Change of Its Characteristics of Tetraploid Muskmelon[J].Northern Horticulture,2012,36(04):104-107.
厚皮甜瓜四倍体的诱导及其特性变化
- Title:
- Induction and Change of Its Characteristics of Tetraploid Muskmelon
- 文章编号:
- 1001-0009（2012）04-0104-04
- Keywords:
- muskmelon; tetraploid; mutation breeding
- 分类号:
- S 652.603.6
- 文献标志码:
- A
- 摘要:
- 以3份二倍体厚皮甜瓜为试材，采用0.2%秋水仙碱处理去掉生长点的幼苗茎尖，研究剥滴法对四倍体诱导率的影响以及四倍体甜瓜特征特性的变化。结果表明：网纹型材料M066的四倍体诱导率达54.4%，光皮型材料M072和M0544四倍体诱导率分别为20.0%和23.3%。与二倍体甜瓜相比，四倍体植株侧蔓节间距变短，花冠与叶片变大，保卫细胞叶绿体数增加，花粉可染率和单瓜种子可育率降低，单果重及果形指数变小，果肉厚度同二倍体，可溶性固形物含量降低。该试验结果说明，不同基因型甜瓜材料诱变率差异显著，四倍体甜瓜生理生态特性变化显著。
- Abstract:
- Three diploid varieties were treated by removing growth point of seedlings and dripping 0.2% colchicines onto the stem tips to induce tetraploid muskmelon．The results showed that the tetraploid mutation rate of netted melon ‘M066’ was 54.4%，that of smooth rind melon such as ‘M072’ and ‘M0544’ were 20.0% and 23.3% respectively．Compared to autotetraploid with original diploid，the length of lateral branch internode was shorter，the size of corolla and leaf were larger，the number of chloroplast per guard cell increased.But the number of perfect seeds per fruit，the stainability of pollen grains，fruit weight and the index of fruit-type decreased．The flesh qualities of soluble solid content declined，but its thickness was same as original diploid．These results demonstrated the mutagenic effects were different according to genotypes，and there were significant changes in physiological and biochemical characteristics between autotetraploid and diploid．
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备注/Memo
第一作者简介：张勇（1971-），男，硕士，讲师，现主要从事西甜瓜育种研究工作。
责任作者：张显（1961-），男，教授，博士生导师，现主要从事瓜类作物遗传与育种研究工作。E-mail：zhangxian@nwsuaf.edu.cn。
基金项目：陕西省科技发展计划资助项目（2011K01-18）；西北农林科技大学唐仲英育种基金资助项目（59）；大学生创新性实验计划资助项目。
收稿日期：2011-11-27