ZHANG Na,SHI Xian-feng,REN Jian,et al.Study on Mutugenesis of Tetraploid Watermelon by Using Oryzalin[J].Northern Horticulture,2013,37(19):52-55.
利用胺磺灵诱变西瓜四倍体研究
- Title:
- Study on Mutugenesis of Tetraploid Watermelon by Using Oryzalin
- 文章编号:
- 1001-0009（2013）19-0052-04
- Keywords:
- small-type watermelon; yellow-flesh; tetraploid; mutugenesis; Oryzalin
- 分类号:
- S 651
- 文献标志码:
- B
- 摘要:
- 为了给无籽西瓜的选育提供优质亲本材料，以小果型西瓜二倍体自交系A8为试材，采用胺磺灵对幼苗生长点进行剥滴处理，设计不同浓度和处理天数，进行四倍体诱导，并进行形态学鉴定、流式细胞仪分析鉴定、保卫细胞叶绿体计数法鉴定，经3代种植观察和筛选，获得了稳定的四倍体材料。结果表明：不同处理均可诱变产生该材料的四倍体，其中最有效的处理是在播种后第8天剥除心叶，再用100 mg/L胺磺灵连续处理裸露的生长点6 d，幼苗成活率达100％，最终诱变率达30.59％。
- Abstract:
- In order to provide breeding high-quality parent material for seedless watermelon，taking small-type diploid watermelon line A8 as material，tetraploid plants of watermelon were induced by treating the growing points of seedlings with different concentration Oryzalin and lasting different treating time.Autotetraploid plants were selected and identified basing on morphology，flow cytometry，hybridization and characterization.Then observed a stable tetraploid material after three quarters’ continuous cultivation，observation and filtering.The results showed that different treatments could obtain tetraploid watermelons by processing with Oryzalin.The most effective way was extirpating interiorleaf at the 8th day after sowing，then treating the growing points of seedlings with 100 mg/L Oryzalin for 6 d.The treatmented seedlings could all survival and the induction frequency of tetraploid watermelon was 30.59%.
参考文献/References:
[1]刘文革.我国无籽西瓜产业发展状况与对策[J].长江蔬菜,2010(8):121-127. [2]王惠林,张慧林,周志成.ORYZALIN不同处理方法对西瓜四倍体诱变效果的影响[J].新疆农业大学学报,2008,31(6):21-25. [3]中国农业科学院果树研究所郑州分所.培育四倍体西瓜初报[J].植物学通讯,1974(2):26-31. [4]袁建民.小果型西瓜离体培养关键技术及四倍体诱导研究[D].海口:海南大学,2010:5-7. [5]王秀莉,赵世萍.秋水仙素诱导细胞周期停滞[J].生物学通报,2010,45(8):51-52. [6]谭素英,刘济伟,黄秀强,等.西瓜新品种‘蜜枚四倍体’的选育研究[J].果树科学,1993,10(2):87-91. [7]祁碧菽,罗耀武,任清.四倍体葡萄花粉母细胞减数分裂与坐果率关系的研究[J].河北农业大学学报,2000,23(4):49-52. [8]施先锋,彭金光,汪李平.用西瓜叶片气孔保卫细胞叶绿体数鉴定西瓜染色体倍性[J].湖南农业大学学报(自然科学版),2009,35(6):640-643.
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备注/Memo
第一作者简介：张娜（1980-），女，硕士研究生，农艺师，研究方向为西瓜栽培与育种。E-mail：zn800329@163.com.