CHEN Rong,ZHU Chang-san.Study on Effect of Precultivation Period on Chromosome Doubling of Alocasia indica[J].Northern Horticulture,2013,37(01):105-107.
预培养时间对观音莲染色体加倍的影响
- Title:
- Study on Effect of Precultivation Period on Chromosome Doubling of Alocasia indica
- 文章编号:
- 1001-0009（2013）01-0105-03
- Keywords:
- Alocasia indica; colchicine; polyploid
- 分类号:
- S 681.9
- 文献标志码:
- A
- 摘要:
- 以观音莲组培苗茎段外植体为诱导材料，采用秋水仙素处理方法进行观音莲的离体诱导，研究预培养时间和共培养时间对诱导效果的影响。结果表明：四倍体诱导率因预培养时间和共培养时间不同而异，在预培养时间为0、3、6、9 d 4个梯度和共培养时间为8、12、16 d 3个梯度组成的12个处理中，切取茎段外植体为诱导材料，在未添加秋水仙素的增殖培养基中预培养6 d,再转入添加秋水仙素120 mg/L的增殖培养基中共培养16 d的处理获得了最大的诱导率和四倍体数目，此时的诱导率达到46.4%；随着预培养时间的增加，外植体成活率及每瓶有效不定芽株数逐渐增加，可见预培养影响外植体的成活、增殖培养及诱导效果。
- Abstract:
- Explants of petiole were taken from in vitro grown plants and to induce polypoids through colchicine treatments.The results showed that tetraploid inductivity was different with different precultivation period and cocultivation period.In 12 cases including four different precultivation periods(0，3，6 and 9 days)and three different cocultivation periods(8，12 and 16 days)，inducing with petiole explants,preculturing in propagation medium without colchicine for 6 days and coculturing in progation medium with 120 mg/L colchicine for 16 days was the most efficient condition for tetraploid induction，yielding highiest inductivity(46.4%).Proplonging precultivation period，explants survial percentage and effective shoots per culture bottle increased gradually.
参考文献/References:
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备注/Memo
第一作者简介：陈荣（1977-），男，广西全州人，博士，讲师，现主要从事植物倍性育种研究工作。E-mail:chentianyigl@126.com.