CAO Jiankang,WANG Xiaohui,PAN Yong,et al.Research Progress on Distant Hybridization Breeding of Paeonia suffruticosa[J].Northern Horticulture,2026,(6):127-134.[doi:10.11937/bfyy.20252433]
牡丹远缘杂交育种研究进展
- Title:
- Research Progress on Distant Hybridization Breeding of Paeonia suffruticosa
- 文章编号:
- 1001-0009(2026)06-0127-08
- 分类号:
- S685.11
- 文献标志码:
- A
- 摘要:
- 牡丹为我国传统名花，具有极高的观赏价值和经济价值。远缘杂交可以有效打破种、属之间的生殖隔离，进而创育出性状优良的种质资源。但牡丹远缘杂交存在严重的不亲和现象，限制了牡丹种质创新的空间。该研究对牡丹远缘杂交育种应用现状进行总结，通过分析远缘杂交不亲和的机理和克服远缘杂交障碍的方法，并对杂种鉴定方法进行梳理，提出以牡丹多倍体育种为切入点，获得更多种质资源作为远缘杂交的桥梁亲本，以期解决牡丹远缘杂交不亲和或亲和性差的问题，为牡丹远缘杂交育种工作提供更加全面的参考依据，加快牡丹种质创新的步伐。
- Abstract:
- Paeonia suffruticosa is a traditional famous flower in China，with extremely high ornamental and economic value.Distant hybridization can effectively break the reproductive isolation between species and genera，thereby creating germplasm resources with excellent traits.However，there is a serious incompatibility phenomenon in peony distant hybridization，which limits the space for peony germplasm innovation.This study summarized the current application status of peony distant hybridization breeding，analyzed the mechanism of distant hybridization incompatibility and the methods to overcome distant hybridization obstacles，and sorted out the methods of hybrid identification.It proposed to take peony polyploid breeding as the starting point to obtain more germplasm resources as bridge parents for distant hybridization，in order to solve the problem of peony distant hybridization incompatibility or poor affinity，provide a more comprehensive reference for peony distant hybridization breeding work，and accelerate the pace of peony germplasm innovation.
参考文献/References:
［1］李嘉珏,张西方,赵孝庆,等.中国牡丹［M］.北京:中国大百科全书出版社,2011.［2］王二强,王晓晖,冀含乐,等.牡丹远缘杂交障碍克服措施探析［J］.特产研究,2022,44(5):115-120.［3］王利民,张和臣,符真珠,等.牡丹花香育种研究进展［J］.分子植物育种,2023,21(12):3998-4005.［4］施丽婷,周鑫洋,叶建丰,等.木本观赏植物远缘杂交育种研究进展［J］.园艺学报,2021,48(9):1827-1838.［5］解玮佳,李世峰,瞿素萍,等.常绿杜鹃不同杂种群间杂交的可育性分析［J］.园艺学报,2019,46(5):910-922.［6］张美玲,陈洪伟,王红利,等.植物远缘杂交育种现状与展望［J］.安徽农业科学,2015,43(1):11-17.［7］邓衍明,叶晓青,佘建明,等.植物远缘杂交育种研究进展［J］.华北农学报,2011,26(S1):52-55.［8］ZHANG K,WANG X,CHENG F.Plant polyploidy:Origin,evolution,and its influence on crop domestication［J］.Horticultural Plant Journal,2019,5(6):231-239.［9］吴婷,贾瑞冬,杨树华,等.蝴蝶兰多倍体育种研究进展与展望［J］.园艺学报,2022,49(2):448-462.［10］段超,张启翔.观赏植物远缘杂交和多倍体育种研究进展［J］.安徽农业科学,2009,37(15):6954-6956,6985.［11］HEBERT C J,TOUCHELL D H,RANNEY T G,et al.In vitro shoot regeneration and polyploid induction of Rhododendron ‘fragrantissimum improved’［J］.HortScience,2010,45(5):801-804.［12］刘思余,张飞,陈素梅,等.四倍体菊花脑与栽培菊种间杂交及F1杂种的遗传表现［J］.中国农业科学,2010,43(12):2500-2507.［13］申状状,李昱樱,荣二花,等.陆地棉和野生斯特提棉种间异源六倍体的合成与性状鉴定［J］.作物学报,2019,45(4):628-634.［14］贾明,董双慧,潮洛濛,等.“四季”青绿苔草多倍体诱导及鉴定［J］.北方园艺,2024(15):52-59.［15］何桂梅.牡丹远缘杂交育种及其胚培养与体细胞胚发生的研究［D］.北京:北京林业大学,2006.［16］BEAN W J.Trees and shrubs,hardy in the British isles［M］.London:J.Murray,1914.［17］马会萍,彭正锋,冀含乐,等.牡丹和芍药的组间远缘杂交育种研究进展［J］.北方园艺,2025(18):131-140.［18］关坤.牡丹亚组间远缘杂交后代的早期鉴定［D］.北京:北京林业大学,2009.［19］JAMES W.Improbable peonies［J］.Horiculture,2003,100(2):44-49.［20］律春燕.黄牡丹野生种与牡丹、芍药栽培品种远缘杂交研究［D］.北京:中国林业科学研究院,2010.［21］郝青,刘政安,舒庆艳,等.中国首例芍药牡丹远缘杂交种的发现及鉴定［J］.园艺学报,2008,35(6):853-858.［22］戴思兰.园林植物育种学［M］.北京:中国林业出版社,2007.［23］宋春花.芍药杂交亲和性的细胞学研究［D］.泰安:山东农业大学,2011.［24］律春燕,王雁,朱向涛,等.黄牡丹与芍药组间杂交花粉与柱头识别的解剖学研究［J］.西北植物学报,2009,29(10):1988-1994.［25］郝津藜,赵娜,石颜通,等.黄牡丹远缘杂交亲和性及杂交后代形态分析［J］.园艺学报,2014,41(8):1651-1662.［26］贺丹,解梦珺,吕博雅,等.牡丹与芍药的授粉亲和性表现及其生理机制分析［J］.西北农林科技大学学报(自然科学版),2017,45(10):129-136.［27］贺丹,王雪玲,高晓峰,等.牡丹芍药远缘杂交亲和性［J］.东北林业大学学报,2014,42(7):65-68.［28］李辛雷,陈发棣,赵宏波.菊属植物远缘杂交亲和性研究［J］.园艺学报,2008,35(2):257-262.［29］刘春,穆鼎,明军,等.百合种间杂交受精前障碍的研究［J］.园艺学报,2006,33(3):653-656.［30］张翔宇,贾文庆,何松林,等.牡丹种间远缘杂交不亲和的细胞学与生理机制研究［J］.林业科学研究,2022,35(4):63-71.［31］刘艳芳.十字花科植物远缘杂交障碍的研究［J］.北京农业,2011(18):66-67.［32］肖佳佳.芍药属杂交亲和性及杂种败育研究［D］.北京:北京林业大学,2010.［33］吕博雅.牡丹芍药远缘杂交不亲和性机理及胚胎败育的研究［D］.郑州:河南农业大学,2016.［34］HAO Q,AOKI N,KATAYAMA J,et al.Crossability of American tree peony ‘High Noon’ as seed parent with Japanese cultivars to breed superior cultivars［J］.Euphytica,2013,191(1):35-44.［35］POLLEGIONI P,OLIMPIERI I,WOESTE K E,et al.Barriers to interspecific hybridization between Juglans nigra L.and J.regia L.species［J］.Tree Genetics & Genomes,2013,9(1):291-305.［36］冯邹荣.遮阴处理对牡丹花期和花色特性的影响［J］.现代园艺,2022,45(18):7-9.［37］陶良如,米银法.外源GA3对“白雪塔”牡丹花期生理效应的影响［J］.北方园艺,2024(20):37-45.［38］盖树鹏,盖伟玲,郑国生.不同条件下牡丹花粉的贮藏寿命［J］.北方园艺,2011(18):104-106.［39］贾文庆,王艳丽,郭英姿,等.大花黄牡丹花粉萌发及贮存特性［J］.林业科学,2021,57(2):82-92.［40］贺丹,张佼蕊,何松林,等.授粉方式对牡丹和芍药远缘杂交受精前障碍的影响［J］.西北农林科技大学学报(自然科学版),2020,48(3):99-106.［41］高小峰.牡丹、芍药组间远缘杂交花粉萌发及胚胎发育的形态学研究［D］.郑州:河南农业大学,2015.［42］郝津藜,董晓晓,袁涛.四种药剂柱头处理对芍药属远缘杂交授粉结实率的影响［J］.中国农业大学学报,2019,24(1):40-45.［43］王旭.芍药属杂交亲和性及硼酸浓度对杂交平均结实数影响的研究［D］.长春:吉林农业大学,2017.［44］CHENG F,AOKI N.Crosses of Chinese and Japanese tree peony and a primary study on in vitro culture of hybrid embryos［J］.Acta Horticulturae,2008(766):367-374.［45］贺丹,苏晓迪,何松林,等.不同培养基对牡丹、芍药杂交种胚培养的研究［J］.山东农业大学学报(自然科学版),2019,50(5):747-752.［46］乔永刚,宋芸.我国园艺植物多倍体育种研究进展［J］.北方园艺,2002(6):7-8.［47］BRANDVAIN Y,HAIG D.Divergent mating systems and parental conflict as a barrier to hybridization in flowering plants［J］.The American Naturalist,2005,166(3):330-338.［48］雷家军，谭昌华，代汉萍，等.草莓五倍体种间杂种的获得及回交研究［J］.吉林农业大学学报,2010,32(3):284-288.［49］WANG A,ZHANG X,YANG C,et al.Development and characterization of synthetic amphiploid (AABB) between Oryza sativa and Oryza punctata［J］.Euphytica,2013,189(1):1-8.［50］高静.秋水仙素诱导牡丹多倍体的初步研究［D］.北京:北京林业大学,2007.［51］程世平,姚鹏强,耿喜宁,等.高温诱导牡丹产生未减数花粉［J］.园艺学报,2022,49(3):581-589.［52］王业云.牡丹杂交后代的形态学和SSR分子标记鉴定［D］.北京:中国林业科学研究院,2016.［53］袁涛.中国牡丹部分种与品种(群)亲缘关系的研究［D］.北京:北京林业大学,1998.［54］刘建鑫.芍药属植物远缘杂交育种与杂交亲和性、F1真实性研究［D］.北京:北京林业大学,2016.［55］陈梦洁.牡丹杂交后代的早期鉴定分析［D］.长沙:湖南农业大学,2021.［56］李保印,周秀梅,张启翔.中原牡丹品种资源的核心种质构建研究［J］.华北农学报,2011,26(3):100-105.［57］吴静,成仿云,张栋.‘正午’牡丹的杂交利用及部分杂种AFLP鉴定［J］.西北植物学报,2013,33(8):1551-1557.
相似文献/References:
[1]郭丽丽,李 军,李金航,等.不同时期盆栽牡丹叶片RNA的提取研究[J].北方园艺,2014,38(12):99.
GUO Li-li,LI Jun,LI Jin-hang,et al.Extraction of Total RNA From Potted Tree Peony Leaves at Different Stages[J].Northern Horticulture,2014,38(6):99.
[2]刘 娜,秦安臣,陈 雪,等.牡丹对B9花期调控的响应研究[J].北方园艺,2014,38(10):56.
LIU Na,QIN An-chen,CHEN Xue,et al.Study on the Peony’s Flowering Regulation Response to B9[J].Northern Horticulture,2014,38(6):56.
[3]杨德翠,郑国生.柱枝孢叶斑病侵染对牡丹生理特性的影响[J].北方园艺,2014,38(01):57.
YANG De-cui,ZHENG Guo-sheng.Effects of Infection by Cylindrocladium canadenseon Physiological Characteristics of Tree Peony[J].Northern Horticulture,2014,38(6):57.
[4]赵兴华,吴海红,杨佳明.百合远缘杂交未成熟子房的离体培养研究[J].北方园艺,2014,38(04):90.
ZHAO Xing-hua,WU Hai-hong,YANG Jia-ming.Study on Lily Distant Hybridization in vitroCulture of Immature Ovary[J].Northern Horticulture,2014,38(6):90.
[5]高宏秀,张莹.牡丹春节催花技术[J].北方园艺,2014,38(06):72.
GAO Hong-xiu,ZHANG Ying.Flower Forcing Techniques of Tree Peony in Winter[J].Northern Horticulture,2014,38(6):72.
[6]刘国彬,兰彦平,曹均,等.板栗和锥栗远缘杂交亲和性研究[J].北方园艺,2013,37(15):1.
LIU Guo-bin,LAN Yan-ping,CAO Jun,et al.Study on the Distant Hybridization Compatibility Between Castanea mollissima and C.henryi[J].Northern Horticulture,2013,37(6):1.
[7]郑玲,程彦伟.六个洛阳牡丹品种解剖结构与抗旱性关系研究[J].北方园艺,2013,37(01):65.
ZHENG Ling,CHENG Yan-wei.Study on Relationship Between Anatomical Structures and Drought Resistance of Some Cultivars of Luoyang Paeonia suffruticosa Andr.[J].Northern Horticulture,2013,37(6):65.
[8]史倩倩,王雁,周琳,等.生根粉及不同基质对牡丹传统品种扦插的影响[J].北方园艺,2012,36(01):91.
SHI Qian-qian,WANG Yan,ZHOU Lin,et al.Influence of Rooting Powder and Culture Substrata on Rooting of Different Traditional Cultivars of Paeonia suffruticosa[J].Northern Horticulture,2012,36(6):91.
[9]王 玮,张旭东,施 江,等.不同品种牡丹萌芽过程中组织抗冷性的变化[J].北方园艺,2013,37(06):52.
WANG Wei,ZHANG Xu-dong,SHI Jiang,et al.Changes of Cold Resistance of Floral Organs During Sprout in Different Tree Peony Cultivars[J].Northern Horticulture,2013,37(6):52.
[10]刘帅,丰震,徐艳,等.牡丹不同品种花期差异的生理机理研究[J].北方园艺,2012,36(03):67.
LIU Shuai,FENG Zhen,XU Yan,et al.Study on the Physiological Mechanism of Flowering Phase Difference in Cutivars of Paeonia suffruticosa[J].Northern Horticulture,2012,36(6):67.
备注/Memo
第一作者简介：曹健康（1997-），男，硕士，助理研究员，现主要从事牡丹种质保护和新品种选育等研究工作。E-mail：974198298@qq.com.责任作者：王二强（1979-），男，本科，高级工程师，现主要从事牡丹种质资源收集、保存、新品种培育及栽培技术等研究工作。E-mail：1078070473@qq.com.基金项目：河南省科技发展计划-中原学者工作站资助项目（224400510002）。收稿日期：2025-07-03