LI Jingjing,RAN Pan,WANG Jia,et al.Tissue Culture and Establishment of Regeneration System of Astragalus membranaceus[J].Northern Horticulture,2024,(14):109-115.[doi:10.11937/bfyy.20234354]
膜荚黄芪组织培养及植株再生体系的建立
- Title:
- Tissue Culture and Establishment of Regeneration System of Astragalus membranaceus
- 文章编号:
- 1001-0009(2024)14-0109-07
- 分类号:
- S 326
- 文献标志码:
- A
- 摘要:
- 以膜荚黄芪幼叶、幼茎和下胚轴为试材，采用在MS培养基中添加不同浓度和不同组合的6-BA、NAA、IAA、IBA和TDZ的方法，研究膜荚黄芪愈伤组织培养和不定芽诱导分化及再生体系的建立，构建膜荚黄芪组织培养及植株再生的完整体系,以期为膜荚黄芪种质资源保护、种苗快速繁育和遗传改良提供参考依据。结果表明：幼茎是诱导愈伤组织的最佳外植体，诱导愈伤组织的最佳培养基为MS+6-BA 1.0 mg·L-1+NAA 0.5 mg·L-1+TDZ 0.2 mg·L-1，诱导率为90.00%；愈伤组织诱导不定芽的最佳培养基为MS+6-BA 1.0 mg·L-1+NAA 0.3 mg·L-1，诱导率为76.60%；幼茎诱导不定芽的最佳培养基为MS+6-BA 1.5 mg·L-1+IAA 0.2 mg·L-1，诱导率为90.30%。生根培养的最佳培养基为1/2MS+IBA 0.5 mg·L-1+IAA 1.0 mg·L-1，生根率为39.30%。
- Abstract:
- Taking the young leaves,stems,and hypocotyls of Astragalus membranaceus as the test materials,different concentrations and combinations of 6-BA,NAA,IAA,IBA,and TDZ were added to MS medium to study the establishment of callus and adventitious bud induction differentiation and regeneration systems of Astragalus membranaceus.The aim was to construct a complete system for tissue culture and plant regeneration of Astragalus membranaceus,in order to provide reference for the protection and utilization of Astragalus membranaceus resources,and laid a technical foundation for the further rapid propagation of seedlings and genetic improvement of Astragalus membranaceus.The results showed that young stems were the best explants for callus,and the best medium for callus induction was MS+6-BA 1.0 mg·L-1+NAA 0.5 mg·L-1+TDZ 0.2 mg·L-1,and the induction rate was 90.00%.The optimal medium for inducing adventitious buds from callus was MS+6-BA 1.0 mg·L-1+NAA 0.3 mg·L-1,and the induction rate was 76.60%.The optimal medium for inducing adventive buds from young stems was MS+6-BA 1.5 mg·L-1+IAA 0.2 mg·L-1,and the induction rate was 90.30%.The optimal medium for rooting culture was 1/2MS+IBA 0.5 mg·L-1+IAA 1.0 mg·L-1,and the rooting rate was 39.30%.
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备注/Memo
第一作者简介：李晶晶(1997-)，女，硕士研究生，研究方向为药用植物资源保护与利用。E-mail:l1210701444@163.com.责任作者：梁文裕（1969-），男，博士，教授，现主要从事药用植物资源保护与利用等研究工作。E-mail:liang_wy@nxu.edu.cn.基金项目：宁夏回族自治区重点研发计划重点资助项目（2022BBF02032）。收稿日期：2023-12-13