YANG Songmin,TANG Ruonan,CHENG Mengya,et al.Study on the Proliferation and Differentiation of Rhizomes in the Hybrid Progeny of Hybrid Orchid ‘Hongmeiren’×Cymbidium kanran ‘Yangguifei’[J].Northern Horticulture,2023,(14):63-70.[doi:10.11937/bfyy.20224732]
杂交兰‘红美人’×寒兰‘杨贵妃’杂交后代增殖与分化研究
- Title:
- Study on the Proliferation and Differentiation of Rhizomes in the Hybrid Progeny of Hybrid Orchid ‘Hongmeiren’×Cymbidium kanran ‘Yangguifei’
- Keywords:
- hybrid orchid; tissue culture; rhizome; proliferation; differentiation
- 文献标志码:
- A
- 摘要:
- 以杂交兰‘红美人’×寒兰‘杨贵妃’杂交后代的根状茎为试材，采用L9（34）正交实验方法，研究了6-BA、NAA、TDZ、不同光周期及不同基本培养基对根状茎增殖、叶芽分化以及花芽分化的影响，以期探究杂交兰根状茎增殖与分化适合的条件。结果表明：在1.0 g?L-1.花宝1号+1.0 g?L-1花宝2号+1.0 mg?L-1 TDZ+1.0 mg?L-1 NAA+0.1 g?L-1活性炭+40.0 g?L-1蔗糖+7.0 g?L-1琼脂+6.0 g?L-1蛋白胨+40.0 g?L-1香蕉+40.0 g?L-1马铃薯处理中，根状茎的增殖效果最好；光周期12 h/12 h（光照/黑暗）的增殖系数最高，10 h/14 h（光照/黑暗）的生长速度最快；在2.0 g?L-1花宝1号+1.0 g?L-1花宝2号+5.0 mg?L-1 6-BA+1.0 mg?L-1 NAA+0.1 g?L-1活性炭+40.0 g?L-1蔗糖+7.0 g?L-1琼脂+6.0 g?L-1蛋白胨+50.0 mg?L-1椰汁的处理中，叶芽分化率最优；在基本培养基MS+5.0 mg?L-1 6-BA+1.0 mg?L-1 NAA+0.1 g?L-1活性炭+40.0 g?L-1蔗糖+7.0 g?L-1琼脂+6.0 g?L-1蛋白胨+40.0 g?L-1香蕉+40.0 g?L-1马铃薯的处理中，根状茎产生了花芽分化。
- Abstract:
- Taking hybrid orchid ‘Hongmeiren’×Cymbidium kanran ‘Yangguifei’ of rhizomes as experimental material，using L9(34) orthogonal experiment method，the effects of 6-BA，NAA，TDZ on different photoperiod and basic culture medium of rhizomes proliferation，leaf bud differentiation and flower bud differentiation were studied，in order to explore the hybrid laingen stems proliferation and differentiation of suitable conditions.The results showed that the best formula of rhizome proliferation,2.0 g?L-1 Huabao 1+1.0 g?L-1 Huabao 2+1.0 mg?L-1 TDZ+1.0 mg?L-1 NAA+0.1g?L-1 activated carbon+40.0 g?L-1 sucrose+7.0 g?L-1 agar+6.0 g?L-1 proteinpeptone+40.0 g?L-1 banana+40.0 g?L-1 potato;the proliferation coefficient was highest at 12 hours/12 hours (light/dark) and the fastest growth rate at 10 hours/14 hours (light/dark).The best formula of leaf bud differentiation,2.0 g?L-1 Huabao 1+1.0 g?L-1 Huabao 2+5.0 mg?L-1 6-BA+1.0 mg?L-1 NAA+0.1 g?L-1activated carbon+40.0 g?L-1 sucrose+7.0 g?L-1 agar+6.0 g?L-1 peptone+50.0 mg?L-1 coconut milk.In MS+5.0 mg?L-1 6-BA+1.0 mg?L-1 NAA+0.1 g?L-1 activated carbon+40.0 g?L-1 sucrose+7.0 g?L-1 agar+6.0 g?L-1 proteinpeptone+40.0 g?L-1 banana+40.0 g?L-1 potato，the rhizome produced flower buds.
参考文献/References:
陈南川,陈少云,陈南东,等.杂交兰新品种‘双艺金龙’[J].园艺学报,2020(S2):3054-3055.[2]洪汗福.杂交兰茎腐病的防治技术研究[D].广州:华南农业大学,2016.[3]曾碧玉,常强.杂交兰品种‘韩国小姐’与墨兰品种‘企剑黑墨’杂交坐果及无菌播种研究[J].热带作物学报,2015,36(3):510-515.[4]姚金澳,王旭承.杂交兰组织培养与快速繁殖技术的分析[J].分子植物育种,2021,19(3):929-936.[5]LI C R,DONG N,ZHAO Y M,et al.A review for the breeding of orchids:Current achievements and prospects[J].Horticultural Plant Journal,2021,7(5):380-392.[6]钟淮钦,林榕燕,黄敏玲,等.杂交兰种质资源遗传多样性的SRAP分析[J].福建农业学报,2016,31(11):1193-1197.[7]ZHOU J Y，BU Z Y,XUE Q,et al.Innovation and prospects of development of flower industry：A case study of Guangxi[J].Asian Agricultural Research,2019,11(1):14-19.[8]LEE J W,OH H G,LEE G J,et al.Breeding of a fragrant，multiflora Phalaenopsis by Neofinetia intergeneric hybrid orchid cultivar，‘Pinky Beauty’[J].Flower Research Journal,2019,27(3):216-221.[9]MULLEN A.Endangered orchids fast tissue culture and cryopreservation[J].Hortscience,2020,55(9):S131.[10]YANG Q,FU Y,WANG Y Q,et al.Genetic diversity and differentiation in the critically endangered orchid(Amitostigma hemipilioides):Implications for conservation[J].Plant Systematics and Evolution,2014,300(5):871-879.[11]姚金澳,王旭承.杂交兰组织培养与快速繁殖技术的分析[J].分子植物育种,2021,19(3):929-936.[12]苏妍.寒兰杂交后代快速繁殖体系及生理生化研究[D].福州:福建农林大学,2016.[13]MEUTLA Z,AVISHEK D,PATCHAREEYA B.Effects of sucrose，carrot juice and culture media on growth and net CO2 exchange rate in Phalaenopsis hybrid ‘Pink’[J].Scientia Horticulturae,2016,205:17-24.[14]叶秀仙,黄敏玲,林榕燕,等.素心建兰无菌播种快繁技术研究[J].福建农业学报,2015,30(10):939-943.[15]陈丹丹.建兰离体培养与形态发生特征研究[D].南京:南京农业大学,2015.[16]漆子钰.周育真,艾叶,等.春兰‘黄梅’ב黄荷’杂交F1根状茎的组培快繁研究[J].福建农业学报,2019,34(9):1032-1039.[17]牛田.春兰种质资源的SRAP分子评价及组织培养[D].泰安:山东农业大学,2014.[18]符红艳,李卫东.国兰的组织培养技术[J].林业与生态,2020(9):25-26.[19]潘银萍.部分国兰品种杂交育种及种胚萌发的研究[D].泰安:山东农业大学,2011.[20]李国平,杨鹭生.若干因素对寒兰根状茎诱导、增殖与分化的影响[J].赤峰学院学报(自然科学版),2019,35(8):26-29.[21]王松太,曾丽婷,方中明,等.兰属植物组织培养研究进展[J].黑龙江农业科学,2019(10):144-151.[22]陈春.建兰‘岭南奇蝶’种子无菌萌发与离体繁殖技术[J].亚热带农业研究,2016,12(2):125-129.[23]谢娟,李龙,邢玥,等.墨兰‘吴字翠’×墨兰‘红花’杂交后代根状茎增殖及芽分化研究[J].热带作物学报,2020,41(5):985-993.[24]刘洋,王玉英,李枝林,等.大花蕙兰‘红酒’×莲瓣兰‘边草素花’杂交种组培快繁技术[J].广西植物,2019,39(10):1327-1333.[25]DANIELA D C,CORREDOR P J P.Differentially expressed proteins in the development of protocormlike bodies of Cattleya tigrina A.richard[J].Acta Physiologiae Plantarum,2020（12）:42.[26]谢析颖.霍山石斛生物钟相关基因克隆及其与培养物糖含量分析[D].福州:福建农林大学,2017.[27]YI T K,KUNG F L.Molecular characterization and expression profile of PaCOL1，a constanslike gene in Phalaenopsis orchid[J].Plants,2020,9(1):68.[28]陈锵.春兰根状茎增殖与诱芽技术研究[J].安徽农学通报,2008(7):33-35.[29]殷丽青,王广东,张建军,等.大花蕙兰(Cymbidium hybridium)离体快速繁殖技术[J].上海交通大学学报(农业科学版),2006(4):365-369.[30]CHITT A R D,HUTOKA Y J.Factors affecting asymbiotic immature seed culture and in vitro propagation of Paphiopedilum insigne (Wall.ex.Lindl.)pfitzer A horticultural important vulnerable orchid[J].Plant Cell Biotecnohlogy and Molecular Biology,2020(21):15-16.[31]KANG H J,KYUNG W,KANG D H,et al. In vitro propagation of Gastrochilus matsuran (Makino) Schltr.an Endangered Epiphytic Orchid[J].Plants,2020,9(4):524.[32]陈昌铭.不同植物生长调节剂对杂交兰生长的影响[J].安徽农业科学,2018,46(10):97-99.[33]漆子钰.春兰（Cymbidium goeringii）组培快繁及其试管开花影响因素研究[D].福州:福建农林大学,2017.[34]JOVANA D,MIRJANA L J,VLADISLAV O,et al. In vitro germination and seedling development of two European orchid species， Himantoglossum jankae Somlyay，Kreutz & óvári and Spiranthes spiralis (L.) Chevall[J].In Vitro Cellular & Developmental Biology,2019,55(4):380-391.[35]王松太,曾丽婷,方中明,等.兰属植物组织培养研究进展[J].黑龙江农业科学,2019(10):144-151.[36]施琴.春剑花梗离体培养褐化防止技术及机理研究[D].成都:四川农业大学,2010.[37]陈进燎,胡庆林,张艺祎,等.寒兰种子无菌萌发及根状茎诱导分化技术研究[J].中国园艺文摘,2016,32(12):16-19.[38]郑立明,庞基.春兰×大花蕙兰杂种试管苗开花现象[J].植物生理与分子生物学学报,2006(3):320-324.[39]龚湉.寒兰成花机理及花期调控研究[D].福州:福建农林大学,2015.[40]刘柳姣,刘震.不同外源激素对墨兰开花结果的影响[J].农业研究与应用,2018,31(5):10-14.[41]陆祖正,罗清.大花蕙兰‘爱神’×连瓣兰‘小雪素’杂交种快繁技术分析[J].分子植物育种,2022,20(11):3737-3744.[42]田英男,张天泽,秦晓杰,等.黑毛石斛×鼓槌石斛杂交种组培快繁技术研究[J].热带作物学报,2021,42(2):356-361.[43]LIANG G,LI F Q,WEN Q L,et al.Explore the effect of exogenous hormones on rapidpropagation of tissue culture of native orchids in northern Guangxi[J].Botanical Research,2017,6(5):275-281.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(14):106.
[4]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(14):80.
[5]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(14):84.
[6]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(14):101.
[7]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(14):89.
[8]杜丽,姚瑶,李勇鹏.‘洛阳包头’大白菜叶柄高效植株再生体系的建立[J].北方园艺,2014,38(04):86.
DU Li,YAO Yao,LI Yong-peng.Establishment of Efficient Plantlet Regeneration System From Petiole Explants for Brassica pekinensiscv.‘Luoyang baotou’[J].Northern Horticulture,2014,38(14):86.
[9]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(14):95.
[10]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(14):106.
备注/Memo
第一作者简介：杨松敏（1998-），女，硕士研究生，研究方向为园林植物与应用。Email：songminyang3199@163.com.责任作者：彭东辉（1971-），男，博士，教授，博士生导师，现主要从事园林植物与应用等研究工作。Email：fjpdh@126.com.kejibu.基金项目：国家自然科学基金资助项目（2019YFD1001000）。收稿日期：2023-02-08