[1]王胜,许琳,刘洋,等.不同培养方式下硒对平菇生长的影响[J].北方园艺,2023,(04):118-124.[doi:10.11937/bfyy.20222941]
　WANG Sheng,XU Lin,LIU Yang,et al.Effects of Selenium on Growth of Pleurotus ostreatus Under Different Culture Methods[J].Northern Horticulture,2023,(04):118-124.[doi:10.11937/bfyy.20222941]
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不同培养方式下硒对平菇生长的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年04 页码: 118-124 栏目: 出版日期: 2023-02-28
Title:
Effects of Selenium on Growth of Pleurotus ostreatus Under Different Culture Methods
作者:
王胜1; 2; 许琳1; 2; 刘洋1; 3; 刘琳玲1; 王寿江4; 闫梅霞1; 2
（1.中国农业科学院 特产研究所，吉林 长春 130112；2.吉林省中药材种植（养殖）重点实验室，吉林 长春 130112；3.吉林农业大学 中药材学院，吉林 长春 130112；4.中农康必硒（北京）生物技术研究所，北京 100081）
Author(s):
WANG Sheng1; 2; XU Lin1; 2; LIU Yang1; 3; LIU Linling1; WANG Shoujiang4; YAN Meixia1; 2
(1.Institute of Special Animal and Plant Sciences，Chinese Academy of Agricultural Sciences,Changchun，Jilin 130112；2.Jilin Provincial Key Laboratory of Traditional Chinese Medicinal Materials Cultivation and Propagation，Changchun，Jilin 130112；3.College of Chinese Medicinal Materials，Jilin Agricultural University，Changchun，Jilin 130118；4.Zhongnongkangbi Selenium(Beijing)Biotechnology Research Institute，Beijing 100081)
关键词:
平菇; 富硒; 硒元素; 培养方式
Keywords:
Pleurotus ostreatus; selenium; selenium element; cultivation mode
DOI:
10.11937/bfyy.20222941
文献标志码:
A
摘要:
以平菇为试材，采用平板培养、液体培养及袋料栽培等不同培养方式，研究了添加不同浓度的硒对平菇生长的影响，以及在液体、袋料培养中平菇对硒的富集能力，探讨3种培养方式的最佳添加硒浓度，以期为平菇栽培的各环节提供参考依据。结果表明：平板培养试验中，硒浓度低于10 μg?mL-1时长势较好，菌丝浓密、整齐，随着硒浓度的增加，菌丝生长缓慢，在1 μg?mL-1时生长速度最快；液体培养试验中，随着硒浓度的升高，菌丝生长受到抑制，平菇菌丝硒含量增加，添加硒浓度为20~30 μg?mL-1时，菌丝体生长量和富硒量最佳。袋料栽培试验中，硒元素添加量对袋料栽培平菇菌丝生长的影响不显著，生物学效率随着添加硒元素浓度的增加而增加，平菇硒含量整体呈现上升趋势，最佳添加硒浓度为100 mg?kg-1，平菇硒含量达到最大，菌丝生长最快，生物学效率最高，且各茬平菇硒含量存在差异。
Abstract:
Taking Pleurotus ostreatus as test material，through the comparison in plate culture，liquid culture and succeeded cultivation under different cultivation way，adding the influence of different concentration of selenium on Pleurotus ostreatus growth，and in the liquid，succeeded to cultivate Pleurotus ostreatus for selenium enriched ability，investigate the best adding selenium concentrations of three training modes，in order to provide reference for each link of Pleurotus ostreatus cultivation.The results showed that when selenium concentration was lower than 10 μg?mL-1，the mycelial grew better and were thick and neat.With the increase of selenium concentration，the mycelial grew slowly，and the growth rate was the fastest at 1 μg?mL-1.In the liquid culture experiment，with the increase of selenium concentration，mycelial growth was inhibited，and the selenium content of Pleurotus ostreatus mycelial increased.When the selenium concentration was 20-30 μg?mL-1，the mycelial growth and selenium enrichment were the best.Succeeded cultivation experiment，the selenium content of succeeded cultivated mushroom mycelial growth was not significant，the influence of the biological efficiency increased with the increase of adding selenium concentration increasing，the mushroom selenium content in the overall rise，the best adding selenium concentration of 100 mg?kg-1，mushroom selenium content was maximum，hyphae grow the fastest，the biological efficiency was the highest，the selenium content of pleurotus ostreatus was different among different stubble.

参考文献/References:

[1]张金霞,陈强,黄晨阳,等.食用菌产业发展历史、现状与趋势[J].菌物学报,2015,34(4):23.[2]张平,郑志安,赵祖松颖.我国食用菌产业发展变化及对策分析[J].北方园艺,2017(22):167-174.[3]COHEN R,PERSKY L,HADAR Y.Biotechnological applications and potential of wood-degrading mushrooms of the genus pleurotus[J].Applied Microbiology and Biotechnology,2002,58(5):582-594.[4]DA LUZ J M R,PAES S A,RIBEIRO K V G,et al.Degradation of green polyethylene by Pleurotus ostreatus[J].PLoS One,2015,10(6):e0126047.[5]NTOUGIAS S,BALDRIAN P,EHALIOTIS C,et al.Olive mill wastewater biodegradation potential of white-rot fungi-mode of action of fungal culture extracts and effects of ligninolytic enzymes[J].Bioresour Technol,2015,189(4):121-130.[6]卯晓岚.中国大型真菌[M].郑州:河南科学技术出版社,2000.[7]崔波,申进文,马杰,等.河南大型真菌[M].西安:西安地图出版社,2002.[8]戴芳澜.中国真菌总汇[M].北京:科学出版社,1979.[9]应建浙,卯晓岚.中国药用真菌图鉴[M].北京:科学出版社,1987.[10]何卓英,吕作舟.珍稀侧耳类富硒液体培养技术的初步研究[J].食药用菌,2007(1):24-27,44[11]郭慧阳,王彤,胡惠萍,等.富集微量元素食用菌的研究进展[J].中国食用菌,2022,41(4):1-8.[12]上海出入镜检验检疫局.出口食品中硒的测定方法：SNIT0860-2016[S].北京：国家质量监督检验检疫局，2016.[13]文晴,刘秋梅,马浩佳,等.石膏添加量对平菇熟料栽培的影响[J].北方园艺,2017(16):166-170.[14]文晴,安运平,魏忠方,等.钾元素对平菇生长发育的影响[J].北方园艺,2018(17):161-165.[15]孔维威,卢娇娇,孔维丽,等.铜对平菇生长发育、品质和铜含量的影响[J].河南农业科学,2017,46(2):83-86.[16]向戌莲,张驰.高等食用真菌集硒特性研究进展[J].湖北农业科学,2019,58(6):5-8,12.[17]尚德静,王关林.富硒深层培养对灵芝生长代谢的影响[J].食品与发酵工业,2000,26(1):29-32.[18]张连富,窦春霞,王莲芳,等.以活化硒矿为硒源的灵芝生富硒研究[J].食品科学,2007,28(6):146-149.[19]陈欣,梁慧星.平菇菌丝富硒培养条件的优化研究[J].食用菌,2009,31(4):6-7,13.[20]赖颖,葛红莲,崔晓弢.响应面法优化灰黑平菇富硒培养条件[J].食品工业科技,2015,36(13):153-156,161.[21]贾蕾,向极钎,殷红清,等.生物活性硒肽的研究进展[J].食品科学,2021,42(15):346-355.[22]HAWKES W C,KELLEY D S,TAYLOR P C.The effects of dietary selenium on the immune system in healthy men[J].Biological Trace Element Research,2001,81(3):189-213.

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备注/Memo

第一作者简介：王胜（1999-），男，硕士研究生，研究方向为灵芝种质资源收集与品种选育及栽培模式探索。E-mail：wangsheng2791@163.com.责任作者：闫梅霞（1982-），女，硕士，副研究员，现主要从事灵芝种质资源收集与品种选育及栽培模式探索等研究工作。E-mail：yanmeixiac@126.com.基金项目：中国农业科学院科技创新工程资助项目（CAAS-ASTIP-2021-ISAPS）。收稿日期：2022-07-18

更新日期/Last Update: 2023-03-30